Analysis Of Of Total Flavonoids Of Toona Sinesis Cultivated In Taihe County And The Study On It's Anti-oxidalivt Activity

Purpose:To optimize the extraction process of total flavonoids of Toona sinensis and to compare the content of total flavonoids in Toona sinensis among five defferent species cultivated in Taihe county.To establish HPLC method for the determination of quercetin in Toona sinensis cultivated in Taihe county. To compare meletin of Toona sinensis among four defferent species cultivated in Taihe county.To determine the content of total flavonoids and nutrient component in fresh and pickled Toona sinensis. To observe effects of quercetin on activity of SOD and MDA of human fetal lung diploid fibroblasts (MRC-5).Methods:1. Designing the orthogonal experiment optimizes Enzyme treatment,by referring the yield of total flavonoids in extraction.Use the best extraction method to extract the total flavonoids from five species of Toona sinensis cultivated in Taihe county,and compare the size of its content.2. The HPLC analysis was performed on a shim-pack:vp-ODS column with methanol-water(1:1,adjust pH to 2.50 by dilute phosphoric acid) as the mobile phase,the detective wavelength was 368nm,the flow rate was 1.0ml·mim-1.3. Using three different methods:ethanol ertraction,ultrasonic ertraction,enzyme treatment to determine the content of total flavonoids in fresh and seven-day pickled Toona sinensis with the yield of total tlavonoid as examine items.using coomassie brilliant blue method, phenol sulfuric acid method and redox titration,with the content of protein, soluble sugar and Vc as examine items,to determine the content change of nutrient component in fresh and seven-day pickled Toona sinensis.4. MRC-5 cell lines were treated with quercetin.Using hydroxylamine ethod and chemical colorimetry method to determine the contents of SOD and MDA.Results:1. Here are the best extraction condition of Enzyme treatment. The dosage of enzyme is 15 U/g, the time of Enzymolysis is 90 min, the time of the extraction after enzymolysis is 120 min, and the ratio between solid and liquid is 1:40. The total flavonoid content of red oil toona is the highest, followed by black oil toona, red toona, green toona, and hairy toona is the least.2. Quercetin at the concentrations of 83.2～187.2μg·ml-1 showed good linearity(r=0.9995). The quercetin content of red toona is the highest, followed by red oil toona, black oil toona, and green toona is the least.3. The analysis show a significantly decrease of protein and and Vc in seven-day pickled Toona sinensis,the decrease of total flavonoids isn't so significantly.;And the increase of soluble sugar is also not so significantly.4. Compared with those in the control group, the activity of SOD increased obviously, and the content of MDA were decreased significantly in the drug group.Conclusion:1. Enzyme treatment is a better method of total flavonoids extraction. The total flavonoid content of Toona sinensis cultivated in Taihe county is relatively high, with very high value.2. The HLP method was simple,sensitive and reliable.lt can be uesd for the content determination of quercetin.3. After be pickled,the total flavonoid and nutrient component in Toona sinensis were varyingdegrees damged,it's nutritional value were significantly lower than fresh Toona sinensis.This experimental data can be a valuable reference for the study of eating and preservation methods of Toona sinensis.4. Quercetin has a strong antioxidant properties.