Study On Tissue Culture For Rapid Propagation And Regeneration Of Chinese Cherry (Prunus Pseudocerasus L.)

Chinese cherry (Prunus pseudocerasus L.) is originated in China cherry genera with the characteristics of self-pollination, early-maturing, high fruit-bearing rate and sweet fruit, but the fruit is smaller than that of sweet cherry. In recent years, Chinese cherry cultivars were gradually grafted with sweet cherry cultivars which resulted in this unique resource suffering loss and damage. So, tissue culture for rapid propagation and regeneration of plant could save and preserve the resources of Chinese cherry, and in addition, could provide the theoretic base for improving the Chinese cherry heredity by genetic engineering techniques.This experiment researched the effects of different factors on organization rapid propagation and plant regeneration of Chinese cherry, such as the different kinds of basic culture medium, different concentrations and kinds of plant growth regulators, the leaf placement ways, different parts of the leaf, the concentrations of ager, and light culture. Meanwhile, the feasible mediums for the stem tissue culture, the adventitious shoot proliferation, and radication culture, and the leaf regeneration systems were researched, the main results are as follows:1. After conventional sterilization, Chinese cherry cultivar'Taixiaohongying'half-lignified shoot explants were cultured on sugar-free MS solid medium for 7 days, and then transferred to the Chinese cherry regular medium with sugar which could reduce well the contamination rate of stem.2. The'Taixiaohongying'half-lignified shoots were used as explants, the best medium for explants growth and induction was: MS + 6-BA 1.0mg/L + NAA 0.1 mg/L, in this medium, new shoots grow rapidly and healthy.3. The best medium for 'Taixiaohongying' proliferation was: WPM + 6-BA 1.0 mg/L + NAA 0.1 mg/L, in this medium, both adventitious bud multiplication and axillary multiplication coexisted, and the proliferation coefficient could be over 8.0.4. The best medium for 'Taixiaohongying' radication was: 1/2 MS + IBA 0.5mg/L, in the medium, the rooting rate is 90%,and the shoot could produce many roots which were long and healthy.5. The better medium and concentration combination of growth hormone for 'Taixiaohongying' callus induction was: WPM + 6-BA 2.0 mg/L + NAA 0.2 mg/L. In the medium, the base of young expanding leaf as explant, with adaxial side down and 5 g/L concentration of agar, were incubated in the dark for 7 days before exposure to light with a 12-h photoperiod, the effect of callus inducement was the best, and the callus induction rate of leaf was 100%. Callus was compact with granular structure and formed globoid, which looked like adventitious buds.