Study On The Laws Of Colonization Of Goose Parvovirus In Breeding Goose's Body Using Fluorescence Quantitative PCR

The Gosling plague, a high contagious disease in the breed of goose, is caused by the Gosling plague virus, and it mainly infects the new born poult. The mortality can reach at 100%. But the incidence rate has intimate relationship with the age of the goose. The incidence rate and mortality will reduce when the age of the goose increase. The aged goose is hardly infected by the virus, but they may carry the virus and then release to the environment as the infection sources. Viruliferous egg kind of goose may transfer the virus to the egg by vertical transmission. In this study, the GPV SP strain was used to find out the selfish gene sequence of the GPV VP3 according to the Genbank. Then the fluorescent quantitative PCR method was developed for the detection of GPV Taq Man based on the specific primer and the probe of Taq Man. The reaction system and reaction condition are optimized in this study, and the standard curve was established to evaluate the specificity and sensitivity. In order to study the field planting disciplinarian of the GPV in the egg kind of goose, we introduce this GPV virus to the goose, take the disease material at the different times, and then use the fluorescent quantitation PCR to detect the existence and content of the virus. The results revealed that 2×103 copy/μL GPV was positive in this study, while the paramyxo viruses, avian influenza virus, and the duck plague virus are all negative. The linearity of the standard curve is excellent, with the correlation coefficient greater than 0.992. It shows that this method has good specificity and sensitivity so it has good practical application. In order to study the distribution of GPV in goose, we detect the virus in different tissues of goose.Result, hypodermic GPV vivi-virus to the aged goose, After injection 24hs, the virus can be detected in the heart,liver,spleen,intestinal tract,kidney,dung,oarium,parastata varicosa. After injection 72hs and 168hs, virus can be detected in the heart,liver,spleen,intestinal tract,kidney,dung,oarium,parastata varicosa. After injection 360hs,virus can be detected in the liver,spleen,intestinal tract,dung,oarium,parastata varicosa. After injection 720hs, virus can be detected in the liver,spleen,dung.This proposed fluorescent quantitative PCR method is specific and sensitive and could be utilized for regular monitoring of GPV routine analysis. The amount of virus is different in different organization, Provide theoretical basis for Clinical application and Prevent the happening of the disease.