Rape Bee Pollen Broken And Characterization Of Assessment

Bee pollen contains proteins, carbohydrates, unsaturated fatty acids, vitamins, minerals and nutritional composition and other features material, this has a high nutritional value, and has anti-aging, beauty, prevent cardiovascular disease, liver , strengthen the role of gastrointestinal function. However, the presence of bee pollen wall hinders the absorption of its internal components, and therefore the broken deal.This study used a complex protease, neutral protease, protease and flavor of protease treatment of broken pollen, from which select the best combination of ultrasonic effect of protease treatment of bee pollen, in order to further enhance the enzymatic effect. And the effects of various enzymes were characterized by broken assessment, evaluation indicators include the value of specific surface area, particle size analysis, Zeta potential, environmental scanning electron microscope. Finally, ultrasound-assisted digestion of samples and blank samples for components, the use of high performance liquid chromatography and gas - mass spectrometry of one of the flavonoids, vitamins and fatty acids were determined.Rape bee pollen by four enzymes, the cell permeability have been improved, the contents of dissolution increased significantly. The optimal protease enzyme which parameters pH = 8, T = 48℃, t = 48h, the optimal neutral protease enzyme parameters pH = 9, T = 55℃, t = 46h, the most protease excellent hydrolysis parameters are pH = 6, T = 53℃, t = 33h, the optimal protease digestion flavor parameters are pH = 7.5, T = 46℃, t = 24h. Characterization of broken effects of various assessment results show that the protease has a significant effect and promote the contents of broken dissolution, of which the best broken protease complex, the treated surface area 12.7688m2 / g, compared with the blank an increase of 145.49% compared; treatment, the average size of pollen 23.747μm, compared with the blank reduce the 7.391μm; treated samples the absolute value of Zeta potential as 24.49mv, the potential of higher than the blank sample 14.79mv; scanning electron microscopy and transmission electron microscopy photos show the intuitive broken bee pollen and contents dissolution.Separate treatment using ultrasound rape bee pollen, bee pollen can be germinated holes along the outer wall broken into three single valve, but no effect on the wall, the contents of dissolution is not obvious. Treating rape bee pollen with ultrasonic protease assisted with ultrasound, ultrasonic breaks extrinsic wall, enzyme breaks inside wall, a combination of both achieve the best results. Process parameters for the ultrasonic power 1000W, ultrasonic 360min, temperature 48℃, pH8, protein solubility index was 73.0564%; specific surface area is 11.450 m~2 / g; average particle size is 17.667μm; processing 360min SEM images of the pollen wall has been basically All the single lobe form; TEM images from the treatment 360min visible, the contents have been dissolved through the wall to the outside.Application of HPLC and GC-MS of rape bee pollen composition before and after testing, the results are processed bee pollen flavonoids, vitamins and fatty acids have markedly improved compared with the blank to prove the role of ultrasound to promote pollen breaking enzyme wall, is conducive to dissolution of the contents, so broken it is an ideal method.