The Taxonomy Of Amanita From Jilin And Shandong Provinces And Detection Of Peptide Toxins

Through to the obserbations of 169 specimens deposited in the Academy of Science (HMAS), Mycological Herbarium of Jilin Agricultural University (HMJAU) and Mycological Herbarium of Ludong University,27 species and 2 variations (include four undetermined species) were conducted to the morphological descriptions, illustrations and line drawings.19 species described from Jilin province (include variation and undetermined):A.muscaria, A. muscaria var. formosa, A.subglobosa, A.imazekii, A.longistriata, A. hemibapha, A.crocea, A.vaginata, A.orienfifulva, A.orsonii, A. flavipes, A.subjunquillea, A.subjunquillea var.alba, A.virosa, A.pallidorosea, A. avellaneosquamosa,3 undetermined species.16 species described from Shandong province:A.esculenta, A.ovalispora, A.pseudovaginata, A.crocea, A.griseofolia, A. vagwata, A.orsonii, A. fritilaria, A.sepiacea, A.subjunquillea, A.virgineoides, A.japonica, A.avellaneosquamosa, and undetermined 2 species. A.pallidorosea, A.rubescens,A.virosa,A.crocea and 3 undetermined species are undiscribed in The molecular biology research were conducted to ten Amanitas exists morphological doubt, Based on LSU sequence for the bayesian analysis. Molecular biology results was basically consistent with the taxonomic results. After sequence alignment in GenBank, construction of Phylogenetic Tree and Morphological research,we know that A.sp.3 from shandong province and HMJAU20443, HMJAU 20469,HMJAU20482 from Jilin province were the same species; A.sp.2 is an approximate species of A.crocea. Specimens A.virosa and A.fritilaria and GenBank known sequence was similar and above to 99%. A.sp.1 failed to enlarge the DNA.Using HPLC for detected the main peptide toxins (α-AMA,β-AMA and PHD) of 13 species 15 Amanitas, and the contents of of them in A.pallidorosea basidiocap, cap, the mixture of stipe and volva were determined separately. The result showed that, A. sepiacea, A.pallisorosea and A.sp.3 containsα-AMA, A.fritilaria, A.sepiacea, A.pallisorosea and A.sp.3 containsβ-AMA; A.griseofolia, A.fritilaria, A.sepisacea, A.oberwinklerana and A.pallidorosea contains PHD. HPLC analysis showed that, the contents ofβ-AMA in A.frililaria (HMLD0332) and A.sp.3 basidiocap can considered to preparation of a raw material.The contents ofα-AMA,β-AMA and PHD in A.pallidorosea were determined, they were 30.3168 mg/g,6.9932 mg/g and 9.9459 mg/g in fruit body, and 44.9573 mg/g,11.0798 mg/g and 11.3025 mg/g in pileus, respectively. The contents ofα-AMA and PHD in the mixture of stipe and volva were 11.6904 mg/g and 7.9775 mg/g, but theβ-AMA was not detected in this part.Can be used as the raw material preparation of toxins. In A.pallisorosea also found the contents of each toxin but contents was different, the contents ofα-AMA in cap was 4.43 times of the contents in the mixture of stipe and volva,β-AMA was mainly exists in cap, the content of PHD in cap still higher than the mixture of stipe and volva.