| In this experiment,27 goats were collected and randomly divided into 3 groups including control, low toxic level and high toxic level groups. In each group, the experiment was repeated 3 times. The goats in both low toxic level and high toxic level groups, were given on schedule with MO6+ from [(NH4)6Mo7O24·4H2O] of 15mg/kg and 45mg/kg concentration (the amount of intake was nomalized to each individual's weight), while the control group were given the corresponding amount of single-distilled water. At the day 0,10,20,30,40 and 50, the blood samples were collected and serum was separated for the test of ALT, TP, CP, ALB, T-AOC, SOD, MDA, NO and NOS. At the day 0,25 and 50,3 goates in each group were put under anethesia and killed, and a small amount of liver were collected for the test of mitochondria's T-AOC, SOD, MDA, NO, NOS and CAT.And a partial liver were collected, formalin fixed for histologic observation. And a precisely measured amount of liver were collected for the molybdenum, copper, ferrum and zinc level test. The results of this experiment can be summarized as follows:(1) In the experimental groups, various symptoms began to show up 10 days after the intake of [(NH4)6Mo7024·4H20], among which the most significant one was the losing of appetite. Following that, the original brown hair on the back turned white, while the moulting became more and more serious; Moreover, the body weight decreased quite significantly, especially with an accelerated rate at the later stage of the experiment. All these indicate the success of reproducing the pathological model for goat's molybdenum intoxication.(2) Through different stages of the experiment, the serum's TP, ALB and CP level of both 15mg/kg and 45 mg/kg group was decreasing compared to those of the control group (P<0.05or P<0.01), but the ALT and AST level of the two was increasing (P<0.05or P<0.01).(3) Through different stages, the. serum's T-AOC and SOD level of the two experimental groups was also decreasing compared to those of the control group (P<0.05or P<0.01), but the MDA, NO, T-NOS and i-NOS level was going up (P<0.05or P<0.01).(4) At day 50, the liver of the two experimental groups began to bleed and die due to the swelling of the liver cell, while the control group at the same time did not show any pathological changes.(5) Through the experiment, There were decrease in the liver mitochondria's T-AOC, SOD and CAT between the two experimental groups and the control group (P<0.05or P<0.01), but the MDA, NO, T-NOS and i-NOS level was increasing (P<0.05or P<0.01).(6) For the two experimental groups, Accumulation of molybdenum in the serum and liver significantly increased at two experimental groups (P<0.01); the copper concentration in the serum was decreasing (P<0.05or P<0.01), while its concentration in the liver was increasing (P<0.05or P<0.01); the Ferrum and Zinc concentration in blood serum was decreasing (P<0.05), while the two in the liver did not show any sigh of change (P>0.05).The above results indicate that high level molybdenum can bring significant damages to the liver of goats. The mechanism of this damage may be the following: the liver cell's membrane is damaged, and the anti-oxidative capacity of liver mitochondrial is decreased, which leads to a concentration of free radical as well as aggradations of Mo in liver. As a result, the absorption of Cu and Fe is inhibited, which can cause a dearth of Copper in the tissue.
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