| Gerbera Cultivar'Liangfen'is very suitable for viewing and admiring as it has big beautiful color and comely straight stem. And it has high cutting-flower efficiency and long growing time in water. Using the rapid reproduction method of Gerbera culturing not only can reproduce a lot of seed-seedling also can avoid the variety-degeneration problem caused by single-reproduction and seed-reproduction; it could be damaged by viruses easily which seriously affects the quality and yield of cut flow-ers.So the study includes isolated culture and virus-free of Gerbera jiamesonii Bolus. The seeond is Preserving excellent idioplasm resource and stability of fine properties, raising its worth of viewing.At first, the receptacle, stem apex and petiole of the non-infected Gerbera plants grown in greenhouse were selected in the study. The explants were growing on MS basic medium with 6-BAandNAA in different concentration.6-BA and NAA were us-ed to induce the explant to produce calli and differentiate buds. The leaf of test-tube plantlet is selected as the explant to reduce infection rates. When test-tube plantlet ro-oting, they were planted on different substrates to select the most appropriate.Secondly, for Gerbera plants grown in greenhouse and test-tube plantlet form receptacle,5 kinds of viruses were detected. They were TBRV, TRV, TMV, CMV and TSWV. Gerbera Cultivar'Liangfen'infect the 5 kinds of viruses easily. The result illus-trated that only could be detected by TMV.Finally, used heat treatment and medium joining to ribavirin, stem apex and med-ium joining to ribavirin culture, receptacle and medium joining to ribavirin, heat trea-tment culture, and stem apex culture for virus elimination by isolated culture. Among the 5 kin-ds of virus-free, medium joining to ribavirin was used on Gerbera Cultivar 'Liangfen' for the first time.The date illustrated that stem apex culture had a good effect for virus-free of Gerbera Cultivar'Liangfen'.
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