Study On Diversity And Temporal And Spatial Distribution Of Arbuscular Mycorrhizal Fungi Of Clonal Plant Phragmites Communis

Arbuscular mycorrhizal fungi(AMF) is a symbiont that formed with plant nutritive root and soil fungi,which distributes abroad in nature.A large number of experiments indicated that AMF can promote soil nutriment circulation and utilize,improve soil physics and chemistry nature,stabilize and improve the soil structure;There is beneficial orantagonistic interaction between AMF and soil microorganisms and AMF can affect the composition and quantity of plant rhizosphere.AMF influencesplant interspecific competition through the useful effect on one host.The species and quantity of AMF can indicate the change of nature or human activity in ecosystem.moreover,AMF has a very important effect on the protect,recover and reconstitution of ecosystem.In this research,we chose three natural environments,such as Nanliuzhuang,Beiliuzhuang,and Baiyangdian of Anxin in Hebei,andexhibited the research of the colonization and diversity of AMF from Phragmites communis with soil factors,in order to offer basis and technique support for recovering and reconstruction of soil and vegetation in the wet land,and understand the effect of clonal plant growth on the activity anddistribution of soil AMF.Main research results are as follows:1. The experiment separate 8 AMF species of 3 genera,5 belong to Glomus,2 belong to Acaulospora,l belongs to Scutellospora.2. Geographical environment, host plants and soil factors affect the diversity and spatialdistribution paterns of AMF. Glomus is a dominant genera, the next in order is Acaulospora, Scutellospora is lower.3. Following the prolong of time each rate of colonization increased significantly.The total colonization is up to above 90% of each plot in October,and the spore density amount to 651/100g soil,expressing that muti-symbiosis is very common between Phragmites communis and AMF,and both have grown stonger closely.4. In May, every rate of colonization and the spore density had no significant difference.In July,the peak of the fungi vesicle, hyphae,total rate of colonization and the spore density all toke place in the plot of Beiliuzhuang.But in october,the peak presented inthe Nanliuzhuang.5. The detection of the experiment showed that the distribution and the colonization had obvious space heterogeneity, and different plot and soil depth had a evident effect on the rate of colonization and the spore density.The rules of varieties of the distribution and colonization were almost that the biggest rate of colonization of AMF vesicle and hyphae was in the 10-20cm section at the same time, and that of the arbuscule distribution was in the 0-10cm section;the biggest total distribution was in 20-30cm section, and the change of the distribution was not the same with the increase of soil depth.The biggest spore density was in 0-10cm layer, and it dropped off with the incr-ease of the soil depth.6. The distribution and colonization were closely related with the soil factors.The hyphae distribution and the spore density had a negative correlation with pH of soil.And the total colonization had a very notable negative correlation with pH of soil.The spore density had a very positive correlation with the content of nitrogm, and the arbuscule colonization had a very notable negative correlation with the available nitrogen.The spore density soil organic matter had a very notable negative correlation with the soil organic matter.The spore density of AMF had a very positive correlation with vesicle colonization, hyphae colonization and the total colonization,but had a very negative correlation with hyphae colonizaion.7. The results suggested that the growth of the colonal plant Phragmites communis has a great influnce on the avtivity and distribution of AMF.Before and after the intrusion of Phragmites communis, the rate of colonization and the spore density of AMF changed significantly in the sample area.