The Molecule Discrimination Of Rana Chensinensis True And False And Sex Discrimination

Rana chensinensis belongs to Chordata, Vertebrata, Amphibia, Anura, Ranidae and Rana in taxomony.Popularly, Rana chensinensis goes by the name of hashima or yellow frog,etc,while it is called frog medically.Rana chensinensis takes on important medical value and hygienical value.All it's drying is medicine,This is recordated in Pen-ts'ao from past dynasties.officinal parts of Rana chensinensis are female oviduct,which is intituled Oviductus Ranae,also intituled Oviductus Fog.It is rare animalMedicinal materials.There are original drying animal and fat peeled(female oviduct) as Oviductus Ranae commodity at market from goods modality.There are similar piece of material Rana amurensis and Rana nigromaculata false or Buffo bufo gargarizans false at market now. Identificate this medicinal materials mainly according to character trait and character physics and chemistry and so on nevertheless.But on account of this medicinal materials is short of obvious modality and special chemicial element ,so it is very difficult to identificate it in practice.Designing five pairs of primer to PCR reaction for this four species on this studying in order to make the illegal monger have no benefits and support consumer rights.According to the differention of result of PCR reaction for this four species and so identificate false.It is a question that awaitly to solute in fish breeding and poultry raising that how to control the proportion of sex.We must know the differention of male and female and their genetic background inorder to solute this question.Besides Amphibians is at middle transitive location at evolution.It is possible that seeing heteromorphic chromosome in advanced animals,but it is not possible in low animals.So that it is possible that find transitive type in Amphibians and provide some academic help for the research of evolution of chromosome.So designing seven pairs of primer and a primer combination on this study to PCRreaction for the male and female of Rana chensinensis.According to the differention of result of PCR reaction for male and female of Rana chensinensis,so that we can Identificate the sex of Rana chensinensis.It is not enough mainly accommodate to macro morphology and chromosomics to analyse genetical relationship of species on the studying of Amphibians system of evolutionary relation .It is possible that study the genetical relationship of Amphibians from DNA molecule because of the instant development of molecular systematics and DNA molecular engineering.Man want to get hold of urgently the information of nucleus DNA more and more to supply data of mtDNA along with the accumulation of the sequence of mtDNA.So clone and sequence the products of PCR reaction in fouth pairs of primer from the four species.Analyse that result in BLAST in hopes of providing molecular reference at discussing the Sex determinate gene of amphibian and phyletic series.