Cytology And Cryopreservation Studies On Free-living Conchocelis Of Porphyra Yezoensis

Mature Porphyra yezoensis thallus from Hongdao's aquaculture area in Qingdao, were cleaned and incubated at 15℃to release carpospore, which would develop into red-brown conchocelis after 20d culture. Red-brown conchocelis was taken as material to observe its cytological characteristics under light microscope and transmission electric microscope, and cryopreserved by vitrification and encapsulation-dehydration. The results follow as:1. According to cell dimension and color the conchocelis was divided into three phages: conchocelis filament, conchosporangial branch, mature conchosporangial. There are distinctions among the three kinds of cells.①Conchocelis filament is slim and green, in the center of cell a star-shaped chromatophore.②When conchocelis filament grew into conchosporangial branch, the width of cell increase and the length decrease, the color of conchosporangial branch is red, but the location of chromatophore does not change.③While in the phage of mature conchosporangial, the color of cell was continuous brown sphericity. The obvious different is the location of chromatophore, from center of cell to sides of cell and the amount and dimension of vacuole increase greatly.2. The transmission electric microscope observation results showed that the main structures of free-living conchocelis include cell wall, nucleus, chloroplasts, thylakoids, endoplasmic reticulation, pyrenoid, mitochondria, vacuoles, floridean starch, lipid body.①From transverse and vertical transmission electric microscope images we could see that conchocelis filament has a thick two-layer cell wall, pectic layer is on outside and inside is fibre layer. On transverse section chromatophore appears U-shaped and thylakoids are composed of a series of parallel lines like annual ring. On vertical section membrane connects with cell wall compactly, there are many...