| Porphyra yezoensis Ueda is an eatable seaweed which has great commercial value. The free-living conchocelis is an important form for phycoculture of Porphya yezoensis. Encapsulation-vitrification, which combines the advantages of encapsulation and vitrification is a new technology of cryopreservation. So far there is no work which has been published on cryopreservation of conchocelis of Porphyra by encapsulation- vitrification.The free-living conchocelis of Porphya yezoensis Ueda 03D was encapsulated in 3% ca-alginate beads with 30‰ NaCl. After loading and dehydration treatment by the plant vitrification solution (PVS), the beads were plunged into liquid nitrogen (LN) for 24h, and then they were thawed, washed and cultured before determining the viability of the conchocelis and the malondialdehyde (MDA), superoxide dismutas (SOD) content in the sample. The main results were as follows:1 Beads were treated with 100% PVS for 2,5,10,15 and 20 min at 4℃. The results showed that the highest rate of viability was obtained with PVS treatment for 5 min, which was 28.1%.2 Beads were loaded with 25% PVS for 0, 2, 5, 10 and 20 min at 4℃, before they were treated with 100% PVS for 5 min and plunged into LN. The loading for 5min gave the best result, which was 42.3%.3 When the beads were treated at 0℃ the viability, which was 47.9%, increased greatly compared with the treatment at 25℃.4 The evaluation of thawing temperature showed that the best thawing temperature was 40℃5 The MDA and SOD content after loading and dehydrating the beads was not changed, while it increased dramatically after storing the beads in LN for 24h. |
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