Studies On Culture In Vitro And Plant Regeneration Of Colour Vein Variation Of Lysimachia Christinae

Lysimachia christinae, a famous medicinal material, is one of the good foreground landscape plants which has been applied in the gardens just now. Using young stem (including stem tip and young stem segment with axil buds or not) and young leaf from a wild type of variation (with colour vein) as the explants in this research, we found the best medium which was appropriate for the rapid propagation in vitro and regeneration in order to give the fandmental technique for the transformation of this variation. The main results were as follows:The rapid propagation of Lysimachia christinae by tissue culture showed that the best explants were stem tip, young stem segment (with axil buds) and young leaf. Adventitious shoots elongating over 9.3mm in length were efficiently obtained (9.73 shoots per explant) from young stem tip on MS media supplemented with 0.1 mg l^-1 NAA together with 3.0mg l^-1 6-BA after 30 days of incubation. The frequency of shoot regeneration could reach up to 97.48%.Adventitious shoots elongating over 11 mm in length occurred from young stem (with axil buds) explants on MS media containing 0.1mg l^-1 NAA together with 3.0mg l^-1 6-BA. The best response in terms of frequency of shoot regeneration (97.48%) and number of shoots per explant (10.25 shoots per explant) was observed after 30 days of incubation. Adventitious shoots (5.0mm in length) were efficiently obtained (2.44 elongated shoots per explant) from young stem (without axil buds) on MS media containing 0.0mg ^-1 NAA and 2.0mg l^-1 6-BA after 30 days of incubation. The frequency of shoot regeneration was 65.00%.Application of 0.0mg l^-1 NAA together with 3.0mg l^-1 6-BA seemed to be the most appropriate for in vitro propagation of Lysimachia christinae (With the back part of leaf lied on media). The highest percentage of adventitious shoots formation (100%, 7.81 elongated shoots per explant) was obtained after 30 days of incubation. Cutting at basal and middle part of leaf (3-5mm in length) was suitable for leaf regeneration.Rooting was induced in 100% of plantlets on 1/2MS media without plant growth regulators. Transplanting ex vitro and acclimatization for a period of 10 days were sufficient to promote establishment of plants in the greenhouse and 100% of the regenerated plants survived.In the variations of leaf arrangement, whorl variation (three leaves) were more stable (with the aberrance rate of 7.18% and reverse aberrance rate of 6.67%) than intergrowth variation (with the aberrance rate of 29.67% and reverse aberrance rate of 89.68%); whorl variation (four leaves), with the aberrance rate of 1.91% and reverse aberrance rate of 100%, was unstable in variations of phyllotaxy. Therefore, whorl variation formed by three leaves was fit for application in the near future.