| The material of this study was based on the original plants of Lysimachia christinae Hance, Recorded in China Pharmacopoeia (2005 edition). Studies on genetic diversity of Lysimachia christinae Hancei were not only for their resources protection, but also for their rational utilization. Genetic diversity in 28 accessions of Lysimachia christinae Hancei originating from Sichuan province was analysised by morphology, isozyme and RAPD markers. The results were as followed:1. On the analysis of the Lysimachia christinae Hancei germplasm's morphologies which were planted on the farms in Sichuan Agricultural University, the results showed that there were 8 agronomic traits including the main stem length, the main stem diameter, and the main stem branches, only the number of the main stem branches had no significant differences and relatively stable performance. The differences of the remaining characters had reached a very significant level and the variation of the different sources is greater than the same sources between Lysimachia christinae Hancei germplasm individuals.2. In order to find the relationship between the eight agronomic traits, the results showed that the yield of the Lysimachia christinae Hance is closely related to all indicators. Therefore it's not scientific to assess the level of production only by the whole-plant fresh weight and dry weight.3. Through the analysis, the eight indicators were divided into two main components. Their contribution rates had reached 84.417 percent. The results showed that the resources of the variation of germplasm were from the leaf weight, leaf area, whole-plant fresh weight and whole-plant dry weight. The cluster analysis was assessed to a variety of quality Lysimachia christinae Hance by Euclidean genetic distance of 8 forms the basis of agronomic traits. The results showed that the Euclidean distance in the 70.9 level for the 4 clusters. However, clustering results were inconsistent with the geographical distribution.4. The genetic diversity and genetic relationship were studied by RAPD molecular markers for germplasm Lysimachia christinae Hance from the molecular level of studies. In the level of species and germplasm, 28 test materials had a total of 107 amplified bands, in which polymorphic bands were 88; accounting for 82.24% .Each primer can be amplified7-15 bands, with an average amplified of 9.2.5. The UPGMA clustering map was attained according to genetic distance and analyzed comprehensively. The clustering results showed the participants of the germplasm materials of Lysimachia christinae Hance had clearly clustering trends according to geographical distribution.6. 28 germplasm Lysimachia christinae Hance in eight regions were detected in this experiment. The highest quercetin content was found in Nanchong City and the lowest in Nanxi Country of Yibin City. The containing of quercetin content in stems and leaves was analyzed comparatively to 11 germplasm of Lysimachia christinae Hance selected randomly. The results showed that the containing of the quercetin content in straw was significantly higher than in stem.7. Studies on morphology, metabolites and RAPD molecular markers from different levels of Lysimachia christinae Hance revealed the genetic diversity. A method could not be replaced by another method, only the combined, integrated analysis could fully reveal the genetic nature of Glycyrrhiza uralensis.
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