| Ipriflavone is one of the derivatives of isoflavone by man-made. It belongs to plant estrogen, and has the estrogen like effect. It can be used as a new medicine to prevent and cure the osteoporosis. IP can be metabolized to several kinds of metabolites in mankind and animal body, such as M â… -Mâ…¦. And M I is the main metabolite in animal. In this study, method named the improved high performance liquid chromatography had been developed for the determination of IP and its main metabolite Mâ… in cage layer samples. Pharmacokinetics of IP were studied in cage layers, this work is to reveal the major metabolites and the biotransformation pathways, the absorption, distribution,metabolism and excretion of IP in cage layers, further to determine the maker residue and target tissues. Also the main pharmacokinetics parameters were obtained. Research about residues in edible tissues and eggs of IP is to know the metabolism of target tissues , and also to estimate a withdrawal time of IP after in-feed administration, meanwhile to establish the method which can be used to control its residues in edible tissues. The present research includes two parts as follows:â… The pharmacokinetics of Ipriflavone in cage layersTwelve 58 weaks-ISA cage layers were given 3mg IP per oral. The serum were detected by the HPLC after 0,0.5,1.0,1.5,2.0,3.0,4.0,6.0,9.0,12.0,24.0,48.0 hours to know the IP concentration. Research the pharmacokinetics of IP in cage layers to reveal the major metabolites and the biotransformation pathways, the distribution, absorption and excretion of IP in cage layers, further to determine the maker residue and target tissues. The plasma IP concentration-time data were consistented with a two-compartment model after oral administration resulted from the 3P87 pharmacokinetics software. The pharmacokinetic parametres: T1/23.10 h, Tmax2.82 h, Cmax 11.91 ng/mL, t1/2 (ka ) 1.10 h, k21 0.04, k12 0.19, AUC 8594.58 ng/mL. |
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