Evaluation Of Allium Germplasms Based On Morphological Characters And Micrpropagation Of Allium Plants

A total of 74 garlic (Allium sativum L.) accessions were collected from various parts of China, Korea and European countries. Each accession was planted at the farm of Vegetable Institute of Zhejiang University. Cluster analysis was used to determine the relationship among three different groups: Chinese, Korea and European countries. The results indicated that the number of bulb and leaf was important components. Four major clusters were revealed by using principal component and cluster analysis procedures, garlic from Korea and European countries was similar with that from China. The results would provide some evidence and reference value for further research on morphological characters and phylogeny of garlic.The in vitro shoot propagation from stem-disc derived cultures of Allium chinense, a vegetatively propagated plant, was investigated in this experiment. Shoots developed into clusters when explants were inoculated on MS media supplemented with various levels of BA in combination with NAA. Media with different levels of BA and NAA gave significant differences in the production of shoot clusters. When the explants of 4-5 shoots with calluses were cultured approximately 17 shoots were produced per cluster for 8 weeks at 1.0 mgl^-1BA and 1.0 mgl^-1NAA. All shoots were rooted 15 days after cultured on MS supplemented with 0.1-1.0 mgl^-1NAA.The survival rate of plantlets under ex vitro condition was 95% in pots filled with a peat: sand (2:1 v/v) mixture after 2 weeks. The explants of 2-3 shoots with calluses without roots were cultured on MS+NAA 0.1 mgl^-1 media to study the effect of different temperature and levels of sucrose on bulblet formation. The results indicated that bulblets more easily formed when the temperature or levels of sucrose was higher range tested in the experiment. Results from this study demonstrated that A. chinense could be in vitro propagated by using multiple shoot clusters derived from stem-disc.