| Ophiopogon japonicus is a Chinese traditional medicine material, and it draws more researchers' attentions for its shade endurance, evergreenness, stronger cold and hot tolerance, fine regrowth trait and ornamental quality. It has been used as a species of ground cover plant in sunshine deficiency areas, and applied extensively at home and abroad in recent years. Because of the blooming market demand, industrial-scale seedling production of Ophiopogon japonicus is necessary. And optimum conditions for the culture such as optimum N, P2O5 and K2O compounding, sunlight intensity and temperature are the key factors for fast seedling propagation of Ophiopogon japonicus.In order to search the optimum conditions for commercial seedling production of Ophiopogon japonicus, growth and physiological responses to fertilizer compounding, different sunlight intensity and temperature were studied using a Japanese dwarf clone in the pot culture.The results showed that the treatments of N2P2K1 or N2P2K2 (N, P2O5 and K2O were added at 32, 20 and 12 (or 24)mg/L to 1/2Hoagland nutrient solution) were more beneficial for the development of Ophiopogon japonicus than other fertilizer compounding. And the optimum ratio of fertilizer for the seedling and plant growth was N: P2O5: K2O = 7:3:8(8.5).Ophiopogon japonicus grew better with the characteristics of higher chlorophyll content and root activity, rapid tillering, lower malondialdehyde (MDA) content in 25-30℃ environment temperature and 25-50% sunlight transmission rate, while photosynthetic photon flux density(PPFD) was 176.3-352.8μmol·m2·s-1.According to the results mentioned above, the optimum conditions for seedling production of Ophiopogon japonicus was that ratio of N, P2O5 and K2O combined with...
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