Study On Changes Of Microsatellite Markers On Chromosome 2 Of Chickens Suffered From The Tumor Of Marek's Disease

Objective Although microsatellite instability (MSI) has been proved to exist widely in the genome of tumor of human, there is no relevant research reports about MSI in animals. This thesis aims to find whether there is change in Marek's's-tumor-related host genome by scanning MSI of chicken's chromosome 2 partially, and to provide basic data for biology of formation and development of Marek's's tumor, genetics in anti-disease breeding, and molecule diagnosis in terms of host genome.Materials and methods Forty four microsatellite markers on the chicken's chromosome 2 were selected and their primers were synthesized. Ten samples of Marek's tumor and homological normal tissues were taken, and amplified by PCR after extraction of DNA. Being selected by means of gelose gel electrophoresis, denaturation polyacrylamide gel electrophoresis were carried out. The results were compared between the stripes from tumor samples with those of homological normal tissues, thereafter MSI and loss of heterozygosisty (LOH) were analyzed.Results There were 8 microsatellite markers showing MSI in 44, except 5 were amplified unsuccessfully. Among these, ABR8, ABR153 and ABR659 displayed a high incidence of 8/10, 7/10, and 8/10 respectively.There were 8 microsatellite markers showing LOH, among these, ABR4, ABR107, and ABR189 displayed a high incidence of 8/10, 4/10, and 6/9 respectively. The rest displayed a low incidence, 1/10 (ABR493 has an incidence of 1/9).The combination of ABR107, ABR4 and ABR8 would cover all the 10 samples (MSI) and 8 samples of 10 (LOH) that were studied, which was thought as a molecule diagnosis method of MSI of Marek's tumor.