| Monkshood(Radix aconiti lateralis preparata) is the daughter root of Aconitum carmichaeli.Debx of ranunculaceous plant, it is one of traditional Chinese medicines(TCM), and posses many pharmacologic functions, such as cardiotonic, enhancing immune ability, anti-neoplasm,anti-cardiovascular disease, anti-inflammatory and analgesic, antiaging. This paper focused on the following points: extract efficiency components from monkshood, optimize the separation condition of high performance liquid chromatography(HPLC), construct the Reverse phase HPLC fingerprint of fresh and prepared monkshood, compare active components of monkshood from different habitats, and primary study on the RAPD fingerprint of it. The results are as follows:1. Determined the optimal extracting condition, ultrasonator with aether– ammonia (20:1,V/V) solution for 20 min, according to compare extracting efficiency of aether– ammonia, chloroform and anhydrous alcohol system to three main alkaloids of aconitine, mesaconitine and hypaconitine, and compare extracting efficiency methods of cold– soaked and ultrasonic.2. Obtained 20 chromatographic peaks whose separation effect is good based on optimized chromatographic condition. Condition has been optimized by comparing separation efficiency of extraction-solution from methyl alcohol-water and acetonitrile-water system and by the method of multi-segment gradient for separation in RPLC. The stability and precision of the optimized conditions have been verified, and the result revealed good predicting precision and separating rate. The optimized multi-segment gradient for separation method shows its superiority in determining the condition of separation since the analytic time is shorter and the separating rate is more accurate.3. Constructed chromatographic fingerprints of 10 components for the first time in China from different habitats of Hangzhong Shanxi province, Jiangyou and an'xian Sichuan province, Yunan province and Guizhou province by new method of multi-segment gradient elution system in RPLC. evaluated the quality by chromatographic peak of aconitine, mesaconitine and hypaconitine as references of relative standard. Studied the difference of fingerprint of 4 germplasms from Hanzhong. Evaluated the quality of 10 components of different monkshood by the method of compare the relative content. The RPLC fingerprint in this paper accord with the requirements of Technique Requirements of TCM Injection Fingerprint Research (Temporary) in the aspects of precision, stability, repeatability. This method would probably be the standard of judging the species and evaluating the qualities of monkshood because of its good repeatability and characteristic, simple, and quickly.4. Selected RAPD primers which posses stable polymorphism based on setting up the reliable technology system of genome DNA extraction and RAPD-PCR amplification of monkshood which is the resource of fuzi. Detected the genetic polymorphism of 4 groups of the resource plant of fuzi by RAPD marker technology, and constructed the DNA fingerprint of monkshood. The bands amplified by the selected primers were clear, and 5-16 polymorphism sites were found. DNA polymorphism can be detected quickly and conveniently by agarose gel electrophoresis. Analyzed RAPD bands and constructed DNA fingerprint of the 4 resource groups, and determined over 120 polymorphism sites by 3 random primers which was selected earlier from 11 random primers. It is suggest that RAPD fingerprint can be used absolutely to identify the resource plant of fuzi.
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