15 Tomato Quality Analysis And DNA Fingerprint

The quality of 15 tomato(Lycopersicum esculentum Mill.) materials were studied in the experiment, trying to analyze tomato quality traits of the weight of each variable by AHP method (Analytic HierarchyProcess) and the application of principal component method. According to the idea of AHP method for comprehensive evaluation , that is, using three kinds of measure conversion prior to the analysis, the variable values were transformed into the positive variable values and the linear evaluation function with coefficient of re-value right scale were analyzed. The materials' DNA was extracted using modified CTAB. The RAPD reaction conditions, the reaction of agarose concentration and the concentration of reagent were optimized for RAPD-PCR amplification. DNA fingerprints of 15 tomato materials were analyzed. The results are as follows.1. According to the quality analysis, the solids content, lycopene content, Vc content of Li2 was the highest, and Li2 had moderate acidity, higher total soluble sugar content and highest integrated score. S-45 had the lowest integrated score.2. The improved CTAB was applied to extract DNA. The ratio of OD260/OD280 was from 1.8 to 2.0 and OD260/OD230 was more than 2. Furthermore, the results of electrophoresis was clear. All above indicated that the DNA was qualified for RAPD analysis.3. Orthogonal experiment design was used for setting up optimized RAPD reaction. Each sample was amplified in a reaction mixture (25μL in total volume) containing 40ng template DNA, 200μmol/L dNTP (deoxynucleoside triphosphate), 1.25U of Taq polymerase, 2.5μL of 10×PCR buffer, 1.5μL of MgCl₂ and 14μL of ddH₂O. The reaction mixture was cycled through the following temperature profiles: 94℃for 5min, 94℃for 30s (predenaturation) ,36℃for 30s,and 72℃for 1min for 40 cycles. The PCR was terminated at 72℃for 7min.4. Both concentrations 1.2% and 1.4% of agarose ccould distinguish various DNA, selection of the test concentration of 1.2% agarose.5. Eight random primers with good polymorphism were screened from 40 random primers. These eight primer were using on the 15 tomato amplified. They produced a total of 42 fragments with 28 polymorphic fragments. The polymorphism rate was 66.7%. It indicated that the tomato cultivars in the RAPD markers had a very narrow genetic background. The primer a3 could separate Li2 and Li3 with other species, the primers a10 could separate L-23 with other materials, the primer a18 could separate 214 # from other species, primers al9 could separate 214 # and 4 # with other species. The primer c4 could separate Huizhu imitation and L-23 from other species, the primer c5 could separate from other species, the primer c7 could separate Li2 and S-24 from other species, the primers c13 could distinguish L-23 . The tested cluster analysis of 15 materials was carried out. 15 materials could be divided into four categories when the coefficient was 0.683.