Studies On Sex Character Of Siraidia Grosvenorii (Swingle)C. Jeffrey By Genetic Marker

Siraidia grosvenorii(Swingle)C. Jeffrey is a species of Siraitia in Cucurbitaceae,and it is a dioeciousm and a speufic economic plant in Guangxi province. Excellent male and female plant are in dire need of cultivation, so studies on sex character of Siraidia grosvenorii by genetic marker could be used to sex identification and breeding of single male and female ones in the wild resource.Random Amplified Polymophic DNA (RAPD) and Isozyme was applied to study Siraitia grosvenorii's sex identification. The cultivar Qingpiguo was used as experimental material in the study. This study is deal with the analysis of 130 random primers by RAPD-PCR amplification of the male and female DNA pool of Siraidia grosvenorii. The results show that 14 random primers indicated the male and female plants difference. In oeder to realize if the 14 random primers was relative with sex character, the second RAPD was amplified about 14 random primers used the single male and female genomic DNA. The results show that only 5 random primers could be showed the polymorphism between male and female plants. The primers(S60,S90,S100,S343,S357) can be used as molecular markers linked to sex character and could be used for the sex identification of Siraitia grosvenorii cultivars.The peroxidase, esterase, superoxide dismutase, polyphenol oxidase and catalase isozyme were analyzed with vertical slab PAGE and specific cytochemical staining. The results shows that isozyme bands have apparent difference between the male and female plants. The number of different band of sex character was above one in each isozyme. It could be concluded that the difference of isozyme bands may be used for sex identification of Siraidia grosvenorii. In addition, the enzyme activity of high yield female plant, low yield female plant and acarpous female plant were compared.