| Kenaf(Hibiscus cannabinus) is the important fiber crop for textile industry and paper manufacture. Molecular markers-assistant breed and genetic map construction is drop behind the major crop.By now ,the genetic map of kenaf has not been reported. Genetic map construction can offer the detail description of kenaf gene stucture, it's not only the important field in kenaf genetic research ,but it's also the theoretical basis for kenaf gene location and gene clone.using SRAP and ISSR markers we constructed the kenaf genetic linkage map in kenaf. The major results are as follow:(1) with an 203 F individuals from the cross of two kenaf lines Ga42 (from gana) and a lian kenaf (from Egypt ). sequence-related amplified polymorphism (SRAP) and Inter-simple Sequence Repeat Polymorphism (ISSR ) were used to construct a genetic linkage map in kenaf.(2) From the kenaf genome, 27 SRAP primer pairs and 5 ISSR primers, which could reveal poly-morphisms between Ga42 and a lian kenaf, were screened out of 19X19 primer combinations of SRAP and 30 ISSR primers .(3) These markers generated 108 polymorphic bands in the F population analysis. The average of polymorphic bands produced by one primer pair was 3．4,and the maximum was 8．(4) using Mapmaker/3．0, a linkage map was constructed, which consisted of 68 SRAP markers and 10 ISSR markers distributed in sixteen linkage groups(L0D≥30) and spanned 1423．9 cM with an average interval of 18．25 cM between markers. each SRAP primer pair could generate 10-50 clear bands with a length range between 100 and 1000 bp. and about 70 % of bands showed strong signal.(5) Among the 108 markers, 58 markers(54 %) segregated aberrantlyagainst the expected 3: 1 ratio.. As to Li et al. some SRAP markers revealed co-dominant alleles in Brassic, but in this study only two co-dominant loci were detected. As kenaf has 18 pairs of chromosomes, In the present study, the kenaf SRAP map includes 16 linkage groups, which may be attributed to small number of markers. |
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