SRAP Analysis Of Genetic Diversity Of Core Kenaf Germplasm

Kenaf is an important fiber crop for textile industry and paper manufacture. The genetic diversity and relationships of 84 representive kenaf varieties from here and abroad, was evaluated according to SRAP markers in which genomic DNAs were amplified with 26 SRAP primers. The 84 kenaf varieties were clustered into different groups according to the similarity coefficient (Nei-li), which represented the genetic relationship of different varieties. Principal components(PC) of ten yield and quality characters in 74 kenaf varieties were analysed. The main results showed as follows:(1) The results of the Principal components(PC) of ten yield and quality characters in 74 kenaf varieties are as follow: the first four PCs, which might be regarded as fiber yield component factor(52.68%), fiber and stem weight proportion factor(10.77%), fiber quality factor(10.77%) and fiber and stem weight proportion factor(9.18%).The cumulative contribution of the four factors was 86.07% among the varieties.(2) At the level of D=25.18, all varieties were clustered into one major group and several single-variety groups. At the level of D=20.08, the major group was clustered into one large group which include 32 varieties, 4 small groups and 5 single-variety groups (ZB90, Bg52-1, Fuhong6, 85-135 and Yueyin 4).(3) 329 DNA bands were amplified with 26 SRAP primers, of which 248 (75.4%) were polymorphic.(4) From the UPGMA cluster based on the genetic similarity (GS), at the level of 8.5, wild species, semi-wild species and cultivaling-varieties were estabilished from the 84 kinds of kenaf varieties respectively. The result showed that wild species have distant relationship from semi-wild species and cultivaling-varieties and their genotypes are quite different with the latter. There is certain genetic differences among different groups of cultivating-varieties. In order to breed new Kenaf varieties, hybridizing parents which have distant relationship should be chose.(5) From the UPGMA cluster based on the genetic similarity (GS) , the study indicated that the genetic difference among the kenaf varieties in China was small comparatively , which can be improved through germplasm innovation. Using the genetic difference to choose hybridizing parents with germplasm renewed to each other could breed new Kenaf varieties faster than traditional method. SRAP markers...