Analyse Of The Number Of Character Of Fructification Of Tomato Whth ISSR On Base Of Expand Of The F₂ Tomato (Lycopersicon Esculentum)

Filtrate and cultivate a kind of tomato which has high turnout hecome the best way to slove the problem . condition develop improve prepare train By identifying the quantitative trait locus, QTLfor resistant genes to rust and wilt and screening resistant germplasm in tomato, we hope to set up a breeding procedure for hign turnout selection and to get more good germplasm for tomato breeding program. The major results in this paper are as follows:1 The method of expand of tomatoUs tomato for matuerial to expand one seed to about ten plant Study the effect of different method of .Investigation the effects of different hormone and the concentration of the hormon on expand of tomato ,and use multy-steps to move the plant from test tube to field. The result show that use NaCIO for 13 min is the best method of disinfection the seed and Za is the best kind of hormone for expand of tomato .the effects of is better than other . The one use Zeation comes more bud ,and the speed is much quick than other incretion . The result show that MS+0.5mg/L ZA+0.1mg/L IAA is the best one for expand tomato, and is mucu better than other one.Use multy-steps to move the plant from test tube to field could make the plant all survive.2 Optimization of Simple Sequence Repeat (ISSR) technique in TomatoWith the meterials of tomato, a protocol of reproducible simple sequence repeat (ISSR) was established for tomato. The main factors influencing ISSR-PCR including the concentration of Mg2+,dNTPs, Taq polymerase, annealing temperature and the cycles were investigated. Establish the techiqu system that with total volume of 20uL for tomato was 20uL of MgCl2(25 mmol/L) + 2.0 uL of PCR Buffer(10×) + 0.5 uL of dNTP(10 mmol/L) + 0.5 uL of Taq E (5 U/ uL) + 1.5 uL of Primer(20ng/ uL) + 3 uL of DNA(10ng/ uL) + 10.5 uL of H2O, and the appropriate amplification procedure was as follows: preparatorily denaturing for 5 min at 94℃, denaturing for 1 min at 94℃, annealing for 1 min at 52℃, elongating for 2 min at 72℃, 35 cycles, and elongating for 5min at 72℃.3 analyse of the pertinence of character of fructification of tomato whth ISSRDistill the DNA of the tomato which is be expanted,and use Primer which is filtrated frist to reaction ISSR-PCR,and then electrophoresis . If the striation is the same with the female parent then we note 0,if the striation is the same with the male parent then we note 1.Us the software of Stat , we can find the marker which is contact closely with the character of fructification .In this study we found marker 51 is close relate with the character of fructification of tomato.