Chemical Constituents Of The Leaves Of Periploca Sepium Bge

Periploca speium Bge belongs to the family Asclepiadaceae, It is distributed in the Jilin, Liaoning, Neimenggu, Hebei pronvinces and so on of China. The roots of this plant have been used in Chinese folk medicine for the treatment of rheumatism, arthritis, bones and muscles-ache, boost up waist and knees, bones and muscles. Scholars have taken the attention of the Periploca speium. since the fifties of the twentieth century. But few chemical constituents or bioactivities of above ground of Periploca speium Bge have been reported. In order to research the chemical constituents and bioactivities of Periploca speium Bge, we have studied the constituents of the leaves of Periploca speium Bge.Through preliminary systematic test on chemical composition of periploca sepium Bge leaves, the result indicated that main constituents were phenols, organic acids, saponins, flavonoids and its glycosides, steroids and triterpenoids; alkaloids, carbohydrates and its glycosides, tannins,cardiac glycosides, lactones , coumarins and its glycosides were possible constituents.Study on bioactivities of extracts from periploca sepium Bge leaves against Mythimn separata(Walker)and Macrosiphoniella sanborni (Gillette) was carried on by bioassay-driven separation. The result showed that ethyl acetate partition was more activity than others. After ethyl acetate partition was isolated in the silica gel column, F3 was more contact poison activity than others, its LC50 standing at 2.9032 mg/mL in 72 hours against Mythimn separata(Walker), and 0.5263 mg/mL in 24 hours against Macrosiphoniella sanborni(Gillette).Seven compounds were isolated from the ethyl acetate extraction and n-butanol extraction seperately by partitioning the ethanolic extraction from the leaves of Periploca sepium Bge. Five of them were identified by chemical methods and by spectral analysis (IR, UV, 1H-NMR, 13C-NMR and FAB-MS), lup-11(12)-20(29)-two ene-3β-ol is a new compound. The known compounds are oleanolic acid, quercetin, 6′-methyl ester of quercetin-3-O-β-D-glucuronide, isoquercitrin, All of them were isolated from periploca Sepium Bge for the first time.