Studies On The Adventitious Roots Culture In Pseudostellaria Heterophylla, Periploca Sepium Bge And Glycyrrhiza Uralensis Fisch

Pseudostellaria heterophylla, Periploca sepium Bunge and Glycyrrhiza uralensisFisch are importent Chinese herbs and are used widely. The adventitious root culturesof P.heterophylla, P.sepium and Guralensis have an importent influence on theproduction of Chinese herbs resources.Adventitious root of P.heterophylla was induced directly from the root explantsand liquid-cultured adventitious root culture was successfully established. Theoptimization of culture conditions in adventitious root of P.heterophylla wasinvestigated. The optimal culture condition we obtained in experiments was:6g L"1inoculum size,1MS salt strength,4%sucrose,20mM NH4+to40mM NO/and1.25mM phosphate source. Gradually scale-up culture from0.5L shake flask to3L and5L balloon-type bubble bioreactor was observed. The biomass of adventitious root inbioreactor was significantly higher than that in shake flask. While the contents ofactive components were a little lower than that in shake flask. The content of totalsaponin in adventitious root was higher that in native P.heterophylla, however, thecontent of polysaccharide was lower than that in filed cultivation taproots.In this study, the dynamics of biomass accumulation, production of periplocin,medium condition and consumption of carbon, nitrogen and phosphate source wereinvestigated in adventitious roots culture of P.sepium in flasks. The biomass andpeirplocin production reached the maximum peak on day24simultaneously. PH inmedium had a decrease tendency at the beginning of culture and then remained stablearound4.0, however, EC declined during the whole culture with the nutrientsconsumed. Sucrose was almost used up on the first12day and glucose and fructoseinceresed at first and then decreased. In case of nitrogen, consumption of ammoniumis faster than nitrate. Phosphate was almost consumed duirng the first8day andslowly reduced to0.Adventitious root of Guralensis was induced directly from the leaf explants andliquid-cultured adventitious root culture was successfully established. Theoptimization of culture conditions in adventitious root of Guralensis was investigated.The optimal culture condition we obtained in experiments was:6g L"1inoculum size,1/2MS salt strength,5%sucrose,10mM NH4+to20mM N03" and1.25mMphosphate source. The content of saponin and flavonoid in adventitious root was lower than that native one. However, the content of polysaccharide was higher thanthat in filed cultivation taproots. Gradually scale-up culture was observed. Thebiomass in bioreactor was significantly higher than that in shake flask. Optimizationof culture conditions in bioreactor was investigated which included culture time,inoculum density and aeration volume. The optimal culture condition we obtained inexpeirments was:40days cul"ture time,1g L1inoculum density and0.4vvm aerationvolume.In the study, the adventitious roots culture of P.heterophylla，P.sepium andGuralensis have been successfully established. We also conducted studies onbioreactor culture which layed a foundation for industrial production of metabolites.