The Application Of HPLC-DAD Fingerprint To The Quality Evaluate Of EMEI's Coptis Chinensis Franch

Objective: To establish HPLC fingerprint for the identification of the Emei's Coptis chinensis Franch.To offer the international quality standard which is needed for the planting of the Emei's Coptis chinensis Franch.Methods: The ultrasonic-surge extraction and other extractions were applied to the preparation of the characteristic extraction of the Emei's Coptis chinensis Franch.Take the content of the characteristic extraction as the guide line to choose the extraction. The HPLC-DAD method was applied to research the fingerprint of the Emei's Coptis chinensis Franch and the conditions of chromatogram.At the best extraction and chromatogram conditions, we researched the stability of the sample solution, the precision of the HPLC,and the reproducibility.Then the comparability of the fingerprint of the Emei's Coptis chinensis Franch was researched.At the best extraction and chromatogram conditions,the fingerprint of the Emei's Coptis chinensis Franch was obtained after the sample solution and the reference substance solution had been tested.Then to analyze the characteristic peak of the fingerprint. Then to research the comparability of the fingerprints of the Emei's Coptis chinensis Franch which yielded from different towns of Emei to study whether there quality was identical. The fingerprint of Emei's Coptis chinensis Franch and Coptis dletoidea C.Y. Cheng et Hsiao were compared to study the reliability of the fingerprint of Emei's Coptis chinensis Franch.Results: Methanol- HC1 ultrasonic-surge extraction is the best extraction,it's content of total alkaloid of the Emei's Coptis chinensis Franch is the most.The fingerprint which characteristic peak is completely separate can be obtained by means of gradient elution.After the research of the methodology of fingerprint ,it can be found that the sample solution of Emei's Coptis chinensis Franch is steady within 48 hours,and the reproducibility of the fingerprint is wonderful.The quality of the Emei's Coptis chinensis Franch which yielded from different towns of Emei is identical. The quality of Emei's Coptis chinensis Franch is quite different with Coptis dletoidea C.Y. Cheng et Hsiao .Conclusion: The HPLC-DAD fingerprint of Emei's Coptis chinensis Franch was established elementary,it could be used to research the quality of the Emei's Coptis chinensis Franch and be used as the quality standard of the Emei's Coptis chinensis Franch from the GAP base.