Studies On The Spread Approaches And Selecting The Sensitive Drugs In Vitro For Eperythrozoonosis Of Dairy Cow

Eperythrozoonosis is a zoonosis and caused by eperythrozoon which parasitizes in surface of akaryocyte, blood plasm and bone marrow. Eperythrozoonosis not only reduced the outcome of capill,milk meat and uberty of animals,but also caused serious clinical manifestation and death.So the disease caused large economical lose. Lots of experts considered that insect stings were the intermediary agent of eperythrozoonosis,but these points of view were lacked of basement of experiment,and the disease was epidemic every season. These points could not explain epidemic features of eperythrozoonosis so we inferred that there were other methods which spread the disease. Although there were a lot of reports about eperythrozoonosis ,these reports centered detection,cure etc. There was not discover any report about route of infection of eperythrozoon,so we chosed the routes of eperythrozoonosis in dairy cow as our topic. Mclaughlin(1991) reported that E.wenyoni could not infect goats,deer and sheeps without splenic,but cattles,dogs and rabbits were sensitive.Forty-eight young healthy dogs which were sure free from Eperythrozoon by blood test were divided into respiration system infection group, hypodermic inoculation group, digestive system infection group and muscle infection group(every group follows control group),every dog of control group and examinational group was inoculated negative blood(normal dairy cow blood), inoculated positive blood(dairy cow blood infected from Eperythrozoon) hrough respiration system, hypodermic inoculation, digestive system and muscle separately.The infection rate of erythrocyte was measured every day,and the physiology index, blood rut and blood biochemical index were measured at the summit of erythrocyte infection rate. The result showed that the disease can transmit through respiration system,bite by mosquito,alimentary canal and wound. Ten cyophoric rabbits which cyesised 24-25 days were selected.It was made sure that there was not eperythozoon in these rabbits,they were divided vertical control group infection group randomly.The result showed that the disease could spread by vertical transmission.The important theoretical gist was given for the prevention and control to Eperythrozoonosis in dairy cow .Recently,there were lots of drugs cureing perythozoonosis,furthermore, there were more than 10 specifics but there were large differences in clinic. According to conditions which we comprehended by clinic,We select five drugs which were doxycycline,hyclate,huashen-long effect-honggong sulfamonomethoxine, berenil, imidocarb, in order to select sensitive drugs for eperythrozoon of dairy cow in vitro.The result showed that the effect of these drugs from high to low were Imidocarb>berenil>doxycycline>hyclate>huashen-long effect-honggong.