Study On The Effect Of Copper Complexes Of Schiff's Base Derived From Lysine On Rumen Digestion And Metabolism Of Sheep

A Copper complexes drived from Schiff′s base of Lysine with three levels was fed sheep to investigate effects on rumen digestion and metabolism.It was showed that the antibacterial activity of the complexess was related to the species of germ. Escherichia Coli, Staphylococcus Aureus (SA) and Salmonella were inhibited by the complexeshad in the in vitro. It seems that the inhibiting rate of the complexes angaist bacteria was concerned with time according to the inhibiting curve.Four male Xinjiang Merino sheep , with 40 Kg of average live weight, fitted with permanent fistula in the rumen and duodenum, were supplemented with three dosages (0, 4, 8g·sheep-1·d-1) of the complexes in three periods (with 4 sheep in every period) respectively to study the effect on rumen digestion and metabolism. The results showed that: The intake of DM of corn straw was increased 12.5% at the level of 8 g·sheep-1·d-1 (P<0.05). The dosage of 4 g·sheep-1·d-1 had no effect on rumen pH value (P>0.05), but that of 8 g·sheep-1·d-1 tended to slow up the decrease of rumen pH value 3 h after feeding. The dosage of 4 g·sheep-1·d-1 did not influence the concentration of ammonia nitrogen (P>0.05), but that of 8 g·sheep-1·d-1 decreased the concentration of ruminal ammonia nitrogen. The ruminal concentrations of acetic and total volatile fatty acid (TVFA) were decreased, however, the propionic acid and butyric acid were increased, and rumen fermentation type was changed from acetic to propionic acid after addition of the Copper complexes derived from Schiff′s base of Lysine. This experiment indicated that the Copper complexes derived from Schiff base of Lysine could regulate the rumen fermentation. The influence on the amount of Protozoa in the rumen was not significant (P>0.05). Supplementing with the levels of 0 g·sheep-1·d-1 and 4 g·sheep-1·d-1, the amount of protozoa increased firstly and decreased gradually. The level of 8 g·sheep-1·d-1 had a tendency to depress protozoa. Supplementation of different dosages of the complexes could increase the rates of apparent disappearance of DM, OM and hemicellulose in the rumen (P>0.05) comparing with 0 g·sheep-1·d-1. The apparent digestibility of cellulose in the forestomach was reduced with the increase of the complexes (dropped 13.0% and 15.8% respectively).CP reaching the small intestine were 95.52-102.29 g·sheep-1·d-1at the three adding levels. There was no difference in MCP reaching the small intestine and the ratio of MCP to CP in the small intesteine. The ratio of MCP to CP reduced 13.9% and 15.1% respectively (P<0.05). Rumen Bypass Protein (RBP),its ratios to CP in the small intestine and CP intake were increased with the increase of the complexes. Feeding the complexes to sheep did not affected the flow of total free essential amino acid (TFEAA) to the small intestine (P>0.05). The flow of total free lysine to the small intestine in group of 8 g·sheep-1·d-1 was improved by 38.0% (P<0.05) and 22.01% (P>0.05) compared with groups of 0 g·sheep-1·d-1 and 4 g·sheep-1·d-1, respectively. The other essential and nonessential amino acids (EAAs and NEAA) were also not affected. Feeding 8 g·sheep-1·d-1 significantly increased the flow of total nonessential amino acids to the small intestine (P<0.05). The flow of free lysine to the small intestine in group of 8 g·sheep-1·d-1 was improved by 20.69% (P<0.05) and 18.59%(P<0.05) compared with groups of 0 g·sheep-1·d-1 and 4 g·sheep-1·d-1,respectively. The results indicated that Copper complexes derived from Schiff′s base of Lysine had a regulating effect on the flow of amino acid to the small intestine.