| This paper mainly studied in vitro culture of Lily, which compareddifferent varieties of Lily in vitro culture through Oriental Lily, AsiaticLily reproductive organs such as scales bulb, bulblet and floral organ. Fivestages of primary research were processed, which included sterilization,induction and differentiation, subculture multiplication, bulblet formation,rooting and transplanting. The results showed as follows:1.The effects on different reproductive organs sterilization were differentwith different sterilant combinations: 15％NaClO 15 min+0.1％HgCl2 15 minwas suitable to sterilited the bulblet of Asiatic Lily; 15％NaClO 10 min+0.15％HgCl2 10 min was suitable to sterilited the floral organs; 0.1％HgCl2.10min+0.15％HgCl2 10 min was suitable to sterilited the squamas.2. There were different frequencies of buds induction and differentiatiationwith squama of different varieties. The frequency of buds differentiation withAsiatic Lily was 67.74％, the average of induced bulblets was 4.48; that ofManissa was 58.82％and 3.487; that of Starfighter was 52.78％and 4.167. Theappropriate medium for induction and differentiation was MS basic medium added6- BA 0.5mg·L-1+KT0.5mg·L-1+NAA 0.3mg·L-1.3. Different part of a squamas gave different responses to the effect ofinduction on the same varieties. The inducting fequencies of middle scales withAsiatic Lily was the highest, inner scales was the second, and outer scaleswas the lowest. The inducting fequencies of outer scales with Manissa Lily wasthe highest, middle scales was the second, and inner scales was the lowest.But the results of Starfighter Lily showed that the highest was outer scales and the lowest was inner scales.4. The optimal hormone Combination for bulblets of Asiatic Lily was 2.0mg·L-1KT and 0.5mg·L1NAA. The induction frequencies of buds was 33.33％.5. There were different hormone categories and concentration combinationson induction and differentiation of floral organ. When the concentration of2, 4-D was 1.0 mg·L-1, it was good for callus induction. When the medium combinedwith 2.0mg·L-16-BA, 0.5mg·L-1KT and 0.1mg·L-1NAA, it was more profit forbuds induction.6. There were obvious difference in the bud differentiation of calli thatinduced from different parts of Lily floral organ, in which showed that thebud differentiation rate from filament was the highest, followed by petal, thelowest was from ovary of Asiatic Lily; while in Manissa Lily, the highest buddifferentiation rate was from filament, followed by style, and the lowest wasfrom petal. For starfighter Lily, the highest bud differentiation rate was frompetal, followed by style, and the lowest was from ovary.7. The optimal combination of two hormone for buds subculture multiplicationof Asiatic Lily was 1.25mg·L-16-BA+0.20mg·L-1NAA; that of Manissa was 1.0mg·L-16-BA+0.25mg·L-1 NAA; that of Starfighter was 1.0mg·L-1 6-BA+0.30mg·L-1NAA.8. At the same concentration level, the cytomin KT was better than 6-BA insound plantlet aspect.9. Different combinations of three hormones gave different responses to theeffect of buds subculture multiplication. The treatment with 6-BA1.0mg·L-1+KT0.6mg·L1+NAA0.2mg·L-1 showed the better effect than the others.10. There were different effects on subculture multiplication of Lily withadditional different nutritional components. Concentration in the range ofcoconut milk (15-20％) was favorable to buds multiplication and plantlet growth.The effect of coconut milk on subculture multiplication was better than thatof potato juice.11. Under 90g·L-1sucrose concentration, the rate of bulblet formation withAsiatic Lily reached 100％; that of Manissa Lily and Starfighter Lily were 45 g·L-1 and 60g·L-1. High concentrations(100-120g·L-1)sucrose was unfavourableto bulblet formation of Lily plantlet.12. Concentration range of cumarin(10-20mg·L-1) could promot bulbletformation.13. The rooting and sound plantlet effect of NAA was superior to the sameconcentration level of IBA or ABT.14. Low concentration range of MET(0.5-1.0mg·L-1) combined with(1.0mg·L-1)NAA, which could accelerate root induction and sound plantlet.15. Different types of matrix mixture according to a fixed percentagebenefited Lily plantlet growth. The optimal translation matrix mixture was 1/4peat +1/4 sand +1/2 humus soil.16. 1/6MS liquid proved the best concentration in leaf-sprinkle fertilizersfor the plantlets translation of Lily. |
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