Tissue Culture Of Lonicera Confusa DC.

A study was made of inducing axillary buds from the stem with buds of Guangxi Lonicera confusa DC. Seven stages of primary research were processed, that was, the ways of sterilization, the choose of basic medium, the dose of phytohormone in initial and subculture medium, effects of subculturing generations concentrations on shoots, rooting and transplantation. The results were as follows:1. The stem was dipped in 75% Alcohol 25s firstly, then it was dipped in 0.1%HgCl₂(with Polysorbate 80) 9min, that was the best way of sterilization. The result indicated that the rate of pollution was controlled below 25%, and the rate of initiative was kept above 60%.2. MS was chosen as basic medium in the following experiment, in which the ability of differentiation was stronger than B₅.3. 6-BA and NAA were used in the initial experiment. It was found that NAA was useless to sprout auxiliary buds of Lonicera confusa DC. Only used 6-BA in the initial experiment, when the dose of 6-BA was between 1.0～2.2mg /L, the sprouting rate of axial buds and the 6-BA density were proportional. 6-BA2.0mg/L was the suitable dispose, which the average of the germinative buds was 5.2, the rate of induction was 63.3%.4. Only used 6-BA in the subculture proliferation experiment, when the dose of 6-BA was 0.5～2.0mg /L, the sprouting rate of buds and the 6-BA density were proportional. 6-BA1.7mg/L was the suitable dispose, which the number of buds was 10.7and the buds were stronger than the other one.5. In the process of subculture, the average of the germinative buds which was the most of all were11.1 in the 4th generation. As the increasing of generation, the average of the germinative buds went down.6. The result showed that IBA could not induce Lonicera confusa DC. to root. The dose of NAA which rooted effectually should control in 0.15～0.25 mg/L. The dose of MET should control in 1.0～2.0 mg/L. NAA0.25mg/L+MET 2.0mg/L was the best treatment which rooted Lonicera confusa DC. effectually.7. After domesticated the rooting plants 30 days long, the survival rate of transplantation with NAA was 66.7%, the survival rate of transplantation with MET was 70.0%, the survival rate of transplantation with NAA+MET was 100%.