Study On The Technique System About Tissure Culture And Rapid Propagation Of Cattleya

Cattleya,halled as "Orchids King",the flowers are elegant,is one of foreign orchids which is the most cultivated in the world now and the most welcomed by people.The flowers have spwcial fragrant,Cattleya is upscale ornamental flower and also the upscale material for curing flower,often appears in jubilation and banquet,not only suitable in embellishment family window,balcony and indoor environment,but also in placing the flower and flower arrangement appreciation in official business activity,is also high quality material in manufacture the bride's holds flower and the decorate marriage vehicle,becomes one of the important flowers of festival and present relatives and friends.Internationally,a Cattleya's cuting flower values dozens of US dollars.But now because the specialized level of production is not high,the production efficiency is low,market selling price is extremely high,Therefore,Cattleya tissue culture research is the key to solving this problem.This experiment takes root stump as explant and adopts the regeneration way of bud clumps to establish a rapid technique system of micropropagation in Cattleya.This experiment main research around the antiseptic of explants,producing cluster buds, propagating cluster buds,propagating excessive growth seedling,strengthening test-tube seedlings,taking roots and transplanting finally.The main contents focus on the following aspects,such as the choice of explants,asepsis methods,basic mediums and raising modes,the combination and density of hormone,addictions,carbon source and the density of active carbon.The Orthogonal Design is adopted to arrange experiment,and all data analysis was dealt with by SAS 9.1.Through above research,this paper tries to filtrate the best technique parameter combination,build up the superior regeneration system in Cattleya, expand the choice scope of explants,thus enhance Cattleya commercial production efficiency,and provide the technical support.The result shows that the effective flow of asepsis method is follow:root stumps→washed by tap water→dipped in the detergent water about 10min and used a soft brush to clean the surface dirt(not except bracteal leaves)→washed about one hour by tap water→putted on the extra-clean desk→dipped in the 70%alcohol about 30 s→washed 3 times by aseptic water→dipped in the 0.2%HgCl₂ 10 min→washed 8 times by aseptic water(5 min every time)→water dried and the bracteal leaves sweeped away→inoculated. Aseptic rate achieves 66.67%.The main factors of initition are BA and basic medium,and take BA5.0mg/L and KC as most superior.The subordination factor is natural addition,and banana putty 100g/L is best.The best inducement medium is KC + BA 5.0 mg/L + NAA 0.05 mg/L + banana putty 100 g/L + active carbon 1.0 g/L + sugar 30 g/L + agar 8 g/L,pH5.5.The inductivity achieves 83.33%equally The main factors of multiplication of bud clumps are the basic medium,BA,NAA,the height of seedling,dosage of agar,cluster density,active carbon and the mode of seedling.,and the basic culture medium selects KC,BA to take 0.5 mg/L,NAA to take 0.2 mg/L to promote to multiplication of bud clumps obviously.Taking the seedling about 2 cm height,cutting vertically at the bottom of seedlings,the dosage of agar 4 g/L,active carbon 1 g/L,and putting 4 seedlings,may promote to multiplication of bud clumps obviously.The effect of carbon source is not obvious.The best multiplication medium is KC + BA 0.5 mg/L + NAA 0.2 mg/L + banana putty 100 g/L + active carbon 1.0 g/L + sugar 30 g/L + agar 4 g/L,pH5.5.The best medium for propagating excessive growth seedling is 1/2 MS + BA 5.0 mg/L + NAA 0.1 mg/L + banana putty 100 g/L + active carbon 1.0 g/L + sugar 30 g/L + agar 8 g/L,pH5.5.The medium for promoting seedling is KC + BA 0.1 mg/L + NAA 0.2 mg/L + GA₃0.2 mg/L + active carbon 1.0 g/L + sugar 30 g/L + agar 8 g/L,pH5.5.The medium for rooting is KC + BA 0.1 mg/L + NAA 0.2 mg/L + GA₃ 0.1 mg/L + active carbon 1.0 g/L + sugar 30 g/L + agar 8 g/L,pH5.5.When transplanting,first,seedlings are forged in the room for 6 days,then transplanted. Sphagna and the mixture of ceramsite and peanut shell is the best transplant media for Cattley, the living rate can reach 100%,meanwhile,seedlings grow strongly,and leaves become bottle green and glitter,leaves' acreage increased.