| Phalaenopsis, hailed as "Orchids Queen", flower sales has been the first place in the past few years, and has been favored by flower lovers. In addition to be used as potted plant in recent years, people use Phalaenopsis as a substantial material of cut flowers, besides common floral arrangement, It's a good material of production of flowers of chest, in a celebration of occasions in the public eye, and used to produce the bride clothing, Further more in the current domestic popular new wave, Phalaenopsis are well received by the majority of consumers, so bringing high-quality Phalaenopsis demand will increase every year. But now because over all level of professional production of Phalaenopsis is not high, and the key technologies of production are weak, those result in higher production costs, low propagation coefficient and inefficient industrial production. In the same time, many expensive varieties are in shortage, low quality and high market prices. Therefore Phalaenopsis tissue culture research is the key to solving this problem.The axillary buds of the Phalaenopsis pedicels were taken as explants to induce bud clumps and this regeneration way of propagation were studied in this experiment. This experiment resolved around the best ways to exterminate germs of explants, producing cluster buds, propagating cluster buds, strengthening test-tube shoots, engending roots and transplanting finally. The main contents focus on the following aspects: the selection of explants, asepsis methods, basic mediums and raising modes, the combination and density of hormone, different addictions, carbon source and different resisting oxidizing agent. The Orthogonal Design is adopted to arrange experiments, and all data analysis was dealt with by SAS. Through the research above, the main effected factors and the suitable medium in each stage were researched fully, then the technique parameter combination were filtrated, the superior regeneration system about Phalaenopsis to expand the choice scope of explants, improve the propagation coefficient, and, thus enhancing Phalaenopsis commercial production efficiency, provide solution to the current market shortage of fine breed Phalaenopsis, and technical support for professional nursery.The result shows that the effective procedure of setting up asepsis shoots is follow: the axillary buds of the Phalaenopsis pedicels→washed by tap water→dipped in the detergent water about 10min and used a soft brush to clean the surface dirt→washed about 30min by tap water→putted on the extra-clean desk→dipped in the 70%alcohol about 30s→washed 3times by asepsis water→dipped in the 0.2% HgCl29min→washed 8 times by asepsis water→soaked in 500mg/LPVP , the beginnin and the end cut 1cm individually→water dried and the bracteal leaves sweeped away→inoculated. The significant factors of inducing cluster buds is BA and natural additions, the subordination factor is NAA.The best inducement medium is MS + BA8.0mg/L + NAA0.5mg/L+ CM15%+ sugar30g/L + agar8.0g/L + citric acid100 mg/L,pH5.4. The main factors of bud lumps multiplication are the basic medium, KT, 2,4-D, carbon source, and the pH of medium, the height of seedling, the mode of seedling, and the kind of the resisting oxidizing agents. The seedlings about 2cm cut vertically at the bottom can obviously improve the multiplication of bud clumps. Citric acid 100mg/L and pH5.1-5.4 also improve the multiplication of bud clumps. The effect of carbon source is not obvious.The best multiplication medium is B5 + KT 10mg/L + 2,4-D 0.2mg/L + banana juice150g/L+ citric acid 100mg/L + sugar 30g/L + agar 8.0g/L, pH5.1-5.4. The best medium for promoting seedlings and rooting is MS + KT 5.0mg/L + NAA 0.6mg/L +banana juice150g/L + citric acid 100mg/L + sugar 30g/L + agar 8g/L,pH 5.4. The best explant for producing PLB is the root tip. Slashing the root tip and culturing seedling are benefit for initiation. The superior medium for PLB is MS + BA 5.0mg/L + NAA 0.5g/L + banana juice150g/L + citric acid 100mg/L + sugar 30g/L + agar 8g/L, pH 5.4.When transplanting, first, seedlings are forged in the room for 6days, then transplanted. Sphagna is the best soilless media for Phalaenopsis, the living rate can reach 100%, meanwhile, seedlings grow strongly, and leaves glitter and all of them are upstanding.
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