Studies On The Analysis Method Of β-Agonists In Animal Matrix

β-agonist is one kind of veterinary. They can enter human body through food. They may lead to food poison and cause harm to human health when they exceed a certain quantity. Therefore, the detection ofβ-agonist residue has been strengthened in all countries of the world.In this paper, on the background of lack of analysis method forβ-agonist residue in animal-derived food, an analysis method forβ-agonist residue in liver and muscle tissues was investigated systematically. The pretreatment method of sample was discussed and the simultaneous detection of fiveβ-agonists using an LC-MS method has been established.During the pretreatment process, extraction ofβ-agonists in sample was carried out by liquid-solid extraction and the extract was cleaned up by C₁₈ SPE cartridges. The effects of extraction solvents and extractions times were investigated and the best extraction conditions have been established. The optimal conditions of solid-phase extraction were also studied. Average recoveries of spiked analytes ranged from 77.2% to 95.6% with low RSD of 1.9% to 8.3%.The conditions of LC-MS were developed for simultaneous detection of fiveβ-agonists in liver and muscle tissues. Under certain conditions,β-agonists could be separated and RSD were ranged from 0.5% to 1.4%. The limits of detection for the method could be reached at 0.2-0.5 ng/g.The analysis of the spiked samples showed that the established pretreatment and LC-MS methods could be applied to analysisβ-agonist residue in these tissues. The results have highly reliability.