The Controlling Effect Of The Non-Starch Polysaccharides Enzyme On Energy Metabolism In Layer Basal Diet

In the current study a set of experiments were carried out in order to 1)investigate the dose-response of four Non Starch Polysaccharide (NSP) enzymes including xylanase,β-glucanase, cellulase and pectase on in vitro Digestive Energy (IVDE) in layer different diets; 2)establish the mathematical model for the formulation of NSP multiple enzymes and IVDE in layer basal diet; 3)probe into the correlation between AME and IVDE after adding NSP multiple enzymes in corn-mixed meal basal diet of layer.In the first experiment, 4 NSP enzymes were selected to add at 9 levels in the in layer corn-soybean meal diet and corn-mixed meal diet, respectively, to investigate the dose-response of the NSP enzyme on the IVDE in layer diet using one-way randomized block design. The result from in vitro method showed that NSP enzyme improved significantly IVDE in layer diets (P<0.05). The capability of four individual NSP enzymes of affecting IVDE in layer corn-soybean meal diet and corn-mixed meal diet ranked respectively in the following order: xylanase>cellulase>β-glucanase>pectase and xylanase>β-glucanase> pectase >cellulase.In the second experiment, using quadratic regress-general rotary design, the selected NSP enzymes were used to establish the mathematical model for the formulation of NSP multiple enzymes and IVDE in layer basal diet through the two-stage in vitro method. The screened optical enzyme formulation gained from the current study included 755 000.6U/kg of xylanase, 703 297.2U/kg ofβ-glucanase, 7 025.0 U/kg of cellulase and 3 125.8U/kg of pectase.In the third experiment, based on the second experiment, 3 kinds of complex NSP enzymes were selected to evaluate the effects on AME of adult cocks fed forcedly corn-mixed meal diet. Using one-way randomized block design 48 adult cocks were divided into 4 treatment groups, 4 replicates each. The result suggested the complex NSP enzymes could improve AME (P<0.01). The close correlation was detected between AME in vivo and in vitro digested energy (IVDE): AME(MJ/kg)=0.978 1×IVDE+1.077 8 (R2 =0.95,P<0.01).