| The gas chromatography method which could determine the concentration of pyrogallol in Serum was established in order to study toxicokinetics of pyrogallol and toxicity to animal body.The toxicokinetics process in goats and clinic pathology in rabbits were studied.The main results were as follows.1. The gas chromatography(GC) method establishment Through optimizing fixed liquid, derived reagent, the injection chamber temperature, the Flame Ionization Detector (FID) chamber temperature, the method of arising temperature and gas velocity of flow, the optimal conditions of gas chromatography were determined: the fixed liquid for 6% polydieth-ylene glycol succinate(DEGS), derived reagent for N,O-Bis-(trimethylsilyl) trifluoroacetamide (BSTFA), column initial temperature for 170℃after 3 min rising to 190℃at the rate 20℃/min, and holding for 3 minutes, then falling for 7 minutes, the injection chamber temperature for 280℃, the FID chamber temperature for 280℃, the flow velocity of nitrogen gas for 40 mL/min, the flow velocity of hydrogen gas for 40 mL/min, the flow velocity of air for 400 mL/min. The endogenesis components of serum did not disturb the measure of pyrogallol by gas chromatography. The reservered time was about 4.3 min. The calibration curve was linear in the concentration range from 16μg.mL -1 to 200μg.mL -1 ( r = 0.9967) . The detection limit was 10μg.mL -1 and the method recoveries were from 80% to 98.71%. The RSD within a day was less than 3.31%and the RSD between days was less than 5.97%, therefore these indexes were accorded with the request for drug measure.2. The study of toxicokinetics The blood was plucked at different time post administrating orally Pyrogallol to goats at the dose of 100 mg·kg-1. The concentration of pyrogallol in Serum was determined by GC. The result of toxicokinetics showed that the toxicokinetics process of pyrogallol in goats was accorded with one compartment open model with absorption factor. The elimination half time (t1/2) was (2.4646±0.6472) h, which indicated that pyrogallol was quickly eliminated in goats body. The half-time of absorption phase(t1/2ka) was (0.6237±0.0516) h. The area under curve(AUC) was (973.7847±82.41)μg·mL-1·h. The maximun time was (1.7181±0.2379) h and the maximum concentration(Cmax) was (173.5979±17.7271)μg·mL-1. 3. The study of Clinic pathology to rabbits The rabbits were administrated orally Pyrogallol at the dose of 100 mg·kg-1and 50 mg·kg-1and 25 mg·kg-1 once a week for four times. Every time, after administrating orally pyrogallol to the rabbits, the blood was plucked from heart on the seventh day. Serum was separated as sample.Then, the rabbits were dissected. The heart, liver, spleen, lung and kidney were collected and made paraffin section for investigation of their pathologic histology. The results of clinic pathology showed that alanine aminotransferase(ALT), aspartate aminotrasferase (AST), blood urea nitrogen(BUN)and creatinine of test groups comparing with that of control group were significantly different or different (P<0.01 or P<0.05). The epithelium cell of renal tubular was degenerated and falling off. Renal tubular was tumid. The renal tubular epithelium cytoplasm was turbid, and renal tubular antrum was cabined. The center veins of liver were dilated and congestive. Liver cells were tumid and degenerated. Spleen sinus had slight gore. Heart muscular fibers and cells were degenerated. Alveolus wall capillary vessels were dilated and congestive. Alveolus wall was infiltrated by macrophage.
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