CpSSR Analysis Of Variation Of Genetic Diversity In Temporal Dimension Of Natural Population Of Pinus Koraiensis In Liangshui National Nature Reserve

With cpSSR method, the variation of genetic diversity in temporal dimension of natural population of Pinus koraiensis in Liangshui national nature reserve was studied in this paper. Total 121 P.Koraiensis samples aged from 101 to 420 years were grouped into 6 age classes. 5 polymorphic loci were obtained from 2 pairs of SSR primers and the percentage of SSR polymorphic loci was 71.43%.With the POPGENE analysis software, the genetic diversity index was calculated and the variation of genetic diversity was analyzed in every age class level. By Nei's index estimated, the average level of genetic diversity within age classes is 0.1985, the average level of genetic diversity among age classes is 0.0164, and the average level of genetic diversity in the population is 0.2149. It can be calculated that the genetic differentiation within age classes accounted for 92.37%, and the genetic differentiation among age classes only accounted for 7.63%.This result showed that the differentiation of genetic diversity is not obvious in age class level, and genetic diversity of P.koraiensis primarily existed within age class. The trend of Shannon and Nei's index among age classes showed that the genetic diversity of P.koraiensis in Liangshui National Nature Reserve didn't fluctuate very much during 320 years. During that period, the natural population of P.Koraiensis has not been damaged by human activities, is a natural succession process, and has the basic stability of the genetic diversity.