Effects Of Vtamin E On The Growth And Anti-oxidation Capacity Of Carp Intestinal Epithelial Cells In Primary Culture

The carp intestinal epithelial cells in primary culture were selected to evaluate effectsof vitamin E on the growth and development and anti-oxidation capacity. Firstly, the MTTabsorbance values, protein content, Na⁺-K⁺-ATPase activities, alkaline phosphataseactivities in the cells and lactate dehydrogenase activities and ammonia concentration inthe culture medium were investigated after vitamin E was added 96 h. 2.5μg/ml of vitaminE concentration significantly increased carp intestinal epithelial cells MTT absorbancevalues (P＜0.05). The results showed that the protein content, protein content/MTT OD,Na⁺-K⁺-ATPase activities, Na⁺-K⁺-ATPase activities /MTT OD in the cells were verysignificantly enhanced by vitamin E concentration (P＜0.01),andthere were significantcorrelation between vitamin E and protein content, Na⁺-K⁺-ATPase activities,Na⁺-K⁺-ATPase activities /MTT OD in the cells (r₁=+0.4810,P＜0.05; r₂=+0.6740,P＜0.01; r₃=+0.6440, P＜0.01).The regression results showed that there were verysignificant quadratic relationship between Na⁺-K⁺-ATPase activities and vitamin E level(R²= 0.4600, P＜0.01).The alkaline phosphatase activities, protein consumption, proteinconsumption/MTT OD in the culture medium was significantly depressed by vitamin Econcentration (P＜0.05). When the vitamin E concentration achieved the maximum (7μg/ml), alkaline phosphatase activities,protein consumption/MTT OD in the cells werevery significantly lower (P＜0.01). Vitamin E on the carp intestinal epithelial cell culturemedium LDH activity, ammonia concentrations were significantly (P＜0.05) orsignificantly (P＜0.01), and there were significantly negative correlation between vitamin Eand ammonia concentration in the culture medium (r=-0.5100, P＜0.05). The results ofcorrelation analysis showed that there were significant correlation between proteinconsumption/MTT OD in the cells and alkaline phosphatase activities in the cells,ammonia concentration in the culture medium (r₁=+0.4710, P＜0.05; r₂=+0.5540, P＜0.01). Secondly, malondialdehyde concentration in the culture medium and anti-superoxideanion activitity,anti-hydroxy radical activitity, reduced glutathione concentration,the ratiobetween reduced glutathione and oxidized glutathione in the cells were investigated.Theresults showed that anti-superoxide anion activitity,anti-hydroxy radical activitity,reduced glutathione concentration,the ratio between reduced glutathione and oxidizedglutathione in the cells were very significantly enhanced and the malondialdehydeconcentration in the culture medium was significantly depressed with the vitamin Econcentration increasing(P＜0.01). The results of correlation analysis showed that therewere very significant correlation between vitamin E level and anti-superoxide anionactivities,anti-hydroxy radical activities in the cells(r₁=+0.5410, P＜0.01; r₂=+0.8100,P＜0.01;),and vitamin E and anti-hydroxyl radical activity was highly significant quadraticregression relationship(R²=0.7440, P＜0.01). There were significant correlation betweenvitamin E level and reduced glutathione concentration in the cells (r=+0.4070, P＜0.05),but negative significant correlation between vitamin E level and malondialdehydeconcentration in the culture medium (r=-0.4450, P＜0.05;).In summary, proper vitamin E can promote carp intestinal epithelial cellproliferation .But vitamin E can promote carp intestinal epithelial cell growth anddevelopment, with the major vitamin E for a single intestinal epithelial cell developmentand improve the function. Vitamin E safeguarded carp intestinal epithelial cells structuralintegrity. Vitamin E also can be passed to remove free radicals, own cells to increasenon-enzymatic antioxidants and reduction of lipid peroxidation to raise carp intestinalepithelial cells antioxidant capacity.