Effects Of Nano-Zinc Oxide On The Growth And Anti-oxidation Capacity Of Mice Duodenum-epithelial Cells And Jejunum-epithelial Cells In Primary Culture

The mice duodenum -epithelial cells and jejunum-epithelial cells in primary culture were selected to evaluate effects of Nano-Zinc oxide on the growth and anti-oxidation capacity.Firstly,the effects of TPEN on growth of mice duodenum -epithelial cells and jejunum-epithelial cells were irtvestigated by MTT.Secondly,the effects of different sizes of zinc oxide on growth of mice duodenum -epithelial cells and jejunum-epithelial cells were investigated respectively.At last,the effects of three sizes of Nano-Zinc oxide on anti-oxidation capacity of mice duodenum -epithelial cells and jejunum-epithelial cells were investigated respectively.The result shows:1.There were no inhibition effects of growth on mice duodenum -epithelial cells and jejunum-epithelial cells when TPEN containing 2,4,6μmol L^-1 respectively.2.Nano-Zinc oxide could promote the growth of intestinal epithelial cells,and there was a clear dose-response.The effects of 10-15nm zinc oxide were better than 50nm and 100nm zinc oxide.The MTT OD,the total protein content,protein retention Na⁺,K⁺-ATPase activities were significantly or extremely significantly more than the control group(P＜0.05) when 10-15nm zinc oxide concentration was from 0.8μg ml^-1 to 6.4μg ml^-1;while its significantly depressed when 10-15nm zinc oxide containing was 12.8μg ml^-1(p＜0.05).The LDH was significantly increased(P＜0.05) when 10-15nm zinc oxide concentration were 6.4μg ml^-1and 12.8μg ml^-1 respectively.The effects of 50nm and 100nm zinc oxide on growth of mice duodenum -epithelial cells and jejunum-epithelial cells were similar.They increased MTT OD,the total protein content,protein retention Na⁺,K⁺-ATPase activities significantly or extremely significantly(P＞0.05) when containing from 0.8μg ml^-1 to 6.4μg ml^-1;and there were no significantly effects on previous indexes when 50nm and 100nm zinc oxide concentration were 12.8μg ml^-1(P＞0.05)while the LDH was significantly increased(P＜0.05).3.Nano-Zinc oxide could promote the anti-oxidation capacity of intestinal epithelial cells,and there was a clear dose-response of three sizes of Nano-Zinc oxide.The effects of 10-15nm zinc oxide were better than 50nm and 100nm zinc oxide.10-15nm zinc oxide significantly or extremely significantly increased GSH-Px activities,SOD activities and GSH concentration when it's concentration from 0.8μg ml^-1 to 6.4μg ml^-1;while depressed them when its concentration was 12.8μg ml^-1.The effects of 50nm and 100nm zinc oxide on anti-oxidant of mice duodenum -epithelial cells and jejunum-epithelial cells were shnilar.They increased GSH-Px activities,SOD activities and GSH concentration significantly or extremely significantly(P＞0.05) when containing from 0.8μg ml^-1to6.4μg ml^-1;and there were no significantly effects on previous indexes when 50nm and 100nm Zinc oxide concentration were 12.8μg ml^-1(P＞0.05).Otherwise,the NO concentration was increased significantly(P＜0.05) when 10-15nm,50nm,100nm concentration was 12.8μg ml^-1 respectively.In summary,Nano-Zinc oxide can promote of mice duodenum -epithelial cells and jejunum-epithelial cells growth and anti-oxidant capacity.These effects are dose-dependent effects.And the effects of 10-15nm zinc oxide were better than 50nm and 100nm zinc oxide.While the high levels of Nano-Zinc oxide has the inhibition effects of growth,and depresses cell antioxidase activities and anti-oxidant capacity.