Studies On Technique Of Tissue Culture And Plant Regeneration In Tritonia Crocata

Tritonia crocata is a kind of ornamental perennial herbage. So far, little has been reported about the protocol of its fast propagation. This paper mainly deals with the study on corms and roots as explants for regenerated plantlets of Tritonia crocata. By means of orthogonal and comparative experiment design, most favorable conditions for plant regeneration were found including basic media, concentrations and types of plant growth regulators. The origin of adventitious buds and adventitious roots are illuminated through histological observation. The results are follows:(1) The top and middle scales of corm with bud points were better than other parts of the scales. Disinfector: 75% ethanol +0.1% solution of HgCl₂, surface-sterilized: 75% ethanol for 30s,followed by a treatment with 0.1% solution of HgCl₂ for 6min.(2) Primary culture: the best initiation medium for bud differentiation: MS (1/2MS) +BA2.0mg/L+NAA0.5 mg/L, the rate of initiation was 86.67%; the best initiation medium for callus induction: MS + BA 1.0mg/L + NAA 0.05mg/L+2,4-D1.0 mg/L, the rate of initiation was 83.33%.(3) Subculture: the best subculture medium for bud subculture was: MS+BA3mg/L+NAA0.5mg/L,the multiplication rate was 8.3;the differentiation rate of the shoots longer than 3cm was 46.10%;the best subculture medium for callus subculture and redifferentiation was: MS+BA1.0mg/L+NAA0.1mg/L, the multiplication rate was 7.33, redifferentiation rate was 72.44%.(4) Rooting culture: 1/2MS+IBA0.5mg/L+NAA0.5mg/L + AC 0.1% was the best rooting medium, in which the rooting rate was 93.33%,the number of roots was 6.7,the average length of root was 2.76cm.(5) Transplanting: transplanting substrate with 1/4sand+1/4 vermiculite +l/2garden soil was the best, where the survival rate could reach 85%,the seedlings grew very well.(6) Histological observation displayed the adventitious buds and roots of Tritonia crocata were both originated in stele.