| The contact action, repellency and fumigation action ofβ-asarone derived from the rhizome of Acorus gramineus against Rhizopertha dominica (Fab.) adult using drug-film method, drug-film selecting in filter paper and paper fumigation in glass jars were reported in this paper.The result showed thatβ-asarone had better bioactivities to R. dominica, adult at 72 h post-treatment, the corrected mortality at 159.2μg/cm2 was 100%, and the mortailty regression line and LD50 of contact action were Y=-2.8938+5.2530x and 31.82μg/cm2 correspondingly. The repellence level of 157.3, 78.65, 39.33, 19.66μg/cm2 were all more thanⅣ.The corrected mortailties at 100.00, 50.00, 25.00, 12.50, 6.25μL/L were all more than 80.00% at 120 h post-treatment by fumigating. The fumigation regression line and LC50 were Y= 3.17+2.75x and 4.62μL/L.In integrated toxicity test, R. dominica adult appeared poisonal symptom just like losing self-determination movement ability and unresponsive at 48 h post-treatment. The mortality of R. dominica adults was enhanced quickly after 72 h treated. The integrated regression line and LD50 were Y=-10.16+7.67x and 94.49 mg/Kg.β-asarone was mixed with wheat to study the inhibition action against R. dominica progeny. The result showed that it had better control effect to R. dominica. At 200.00 mg/Kg and 150.00 mg/Kg treatments, the corrected mortalities were 100.00% and 96.67% respectively at 7 d post-treatment. The inhibition rate to progeny were 100.00% and 98.84% respectively at 60 d post-treatment. The damage rates to wheat were 0.00% and 1.33%, which were markedly lower than the damage rate (5.00%) of Malathion treatment.Several kinds of important enzymes in R. dominica adult were studied after treated withβ-asarone. The result indicated that enzymes activities represented stronger time-effection. Acetylcholinesterase(AChE) and Glutathione-S-transferase (GSTs) activitives were inhibited byβ-asarone with the prolongling of exposure time at 94.49 mg/Kg dosage. Whereas, the activities of Carboxy lesterase(CarE) and Esterase isoenzyme(EST isoenzyme) were induced byβ-asarone at the same dosage.When R. dominica adult was treated withβ-asarone at 67.5, 80.0, 100.0, 133.3, 200.0 mg/Kg dosages, the activites of AchE and GSTs were induced at 67.5 mg/Kgβ-asarone and inhibited at 200.0 mg/Kg dosage. The CarE activity was enhanced gradualy with the increasing ofβ-asarone dosage at 24 h post-treatment. Some EST isoenzyme bands were strengthened. However, bands of EST isoenzyme at different dosages did not show marked dosage-effection.
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