Studies On Active Ingredients Against Plutella Xylostella L. And Extract Technology In Julans Regia Leaves

Two compounds were isolated from the dried leaves of Julans regia.There chemcial structures were elucidated as juglone(1), kaempferol(2),respectively, by means of spetroscopic analysis(UV, IR, HPLC-MS, 1^HNMR),chemical reaction,and compared by the spectral data of the known compounds.Extraction technique studies had shown:4%NaOH and 70% ethanol solution extract material had better poison to Plutella xylostella L. than other soution's, and 48h Correction mortality: 55.2±3.2%, 58.2±2.2%.The supersonic extract method got most active components, when the original ingredients to the content 100mg/mL and 48h Correction mortality was 67.5±3.1%.But the drench-wash extract method obtained more ointment from Julans regia leaves(Ointment yield:4.13±0.043%).Bioassay in lab, the 70% ethanol extract material and compound 1 had shown high poison to Plutella xylostella L.and Aphis gossypii glover, the 70% ethanol extract showed excellent fungicidal activities against Rhizoctonia solani Kuhn, Sclerotinia sclerotiorum de Bary, Fusariume graminrarum Schw.(Ec₅₀:11.72mg/mL, 14.08mg/mL, 29.6mg/mL) ,weak effect against Fusarium graminearum Schw, Fusarium oxysporun f. sp. Lycopersici and Fusarium oxysporum Schl. f. sp.,and inactive to remain stains.Compound 1 showed excellent pesticidal activities against Plutella xylostella L (48h Correction mortality:90.0% at dosage of 7.5μg/ml) and Aphis gossypii gloverr(48h Correction mortality:70.5% at dosage of 15μg/ml).And LC₅₀ values (48h)was 2.01μg/ml against Plutella xylostella L.Compound 2 showed excellent fungicidal activities against Rhizoctonia solani Kuhn and Fusarium graminearum Schw, Ec₅₀ was 46.89μg/mL and 56.23μg/mL.