Fertility Identification On Crossing Progeny Of Male Sterile Lines Of Alfalfa

It is a very urgent problem to find male sterile lines of alfalfa that have fine genetic characters in seed production of crossing. In this paper, male sterile line of alfalfa was used to be improved with crossing, because the seed production of crossing is low. The affecting factors of seed production of male sterile line of alfalfa and its crossing progenies that are crossed with male sterile lines of alfalfa and good alfalfa that male is fertile were analyzed. The characteristics of female reproductive organs of male sterile line and its crossing progenies are analyzed in cell morphology. The genetic difference of male sterile line and its crossing progenies are analyzed in enzyme protein. The results showed:1,Rate of podding of nature crossing and fecundity of artificial crossing of the male sterile plant BC1-2 that is the crossing progency of male sterile line of alfalfa and Medicago varia Martin.cv.'Caoyuan No.1'is higher than male sterile lines. Rate of podding of nature crossing and fecundity of artificial crossing of the male sterile plants BC1-19,BC1-21,BC1-22,BC1-23 that are the crossing progencies of male sterile line of alfalfa and Medicago sativa L.cv.'Xinjiang Daye'is higher than male sterile lines.2,The pistil characters of the male sterile plants BC1-2,BC1-19,BC1-21,BC1-22,BC1-23 are improved. Hairy cells of stigma of the male sterile plants enwrap loose mastoid cells. The area of mastoid cells exterior is larger. Hairy cells of stigma of the male sterile plants are longer and abduction.Their Style is larger and wide. The cubage of the epidermal cell is larger and the arrangement of passage cells is tight.3,The patterns of isozyme of the male sterile plants and the male fertile plants are obviously difference. The activity of POD isozyme of leaves of the male sterile plants of the crossing progencies is higher than that of the male fertile plants.The bands number and activity of POD isozyme of flowers of the male sterile plants of the crossing progencies are higher than that of the male fertile plants. The bands number of EST isozyme of flowers of the male sterile plants of the crossing progencies are higher than that of leaves.4,The male sterile plants of the crossing progencies have the characteristic bands of POD and EST isozymes of male sterile lines of alfalfa.