Isolation And Identification Of Effective Bacteria In Aerobic Composting Of Poultry Manure

With the development of stockbreeding industry, production of manure for livestock andpoultry has become a social and environmental issue. Aerobic Compost of animal manure as areliable resource is a promising technology to deal with these problems. Because of it's economical,convenient, no more extender energy, no secondary pollution and so on, aerobic compost alreadybecome the main approach in treating the poultry manure in our country. Through this approach wecan turn the manure into some stable and harmless product such as fertilizer to gain more benefitfrom the waste. Following the construction of the agriculture adjusted, centralized and intensiveanimal husbandry also bring more pollution, however, the aerobic compost need such long timethat is problem for us to resolve. Although inoculation can change the microorganism communitystructure, accelerate the compost, improve the quality of the compost product, meanwhile it makesthe microorganism community structure of aboriginal microbes break down which can decrease theeffect of the degradation of organism. To know more about the aboriginal microbes becomes thekey point to resolve this problem. We seldom see the article mentioned about the aboriginalbacterium, although much had been done about inoculation at home and abroad. This study gives aspecial-purpose research on the bacterium by the isolation, purification, degradation ofMacromolecules, Corelativity among the different strains to get the effective bacterium, and thentest their inoculation effection after identification. The main results as follow:(1) Isolation of the bacterium: 97 strains in the whole period of compost, 37 in the calefactivephase, 31 at the high-temperature phase, and 29 in the depressed-temperature phase.(2) Through the experiment of macromolecules (such as starch, fat, protein, cellulose)degradation we can get 17 effective bacterium, 6 in the calefactive, 4 at the high-temperature, and 7in the depressed-temperature phase.(3) Bacteria can be isolated from every phases during the whole aerobic compost. Theconcentration of Bacteria changes obviously with temperature and exists two peaks. Then decreasemildly at the end of the compost.(4) Aboriginal effective bacteria in the aerobic compost are Bacillus, Pseudomonas,Sporolactobacillus, Micrococcus, Enterobacteriaceae, Cellulomonas, Proteobacteria, Edwardsiellaand so on. Bacillus, Pseudomonas, Sporolactobacillus, Micrococcus, Proteobacteria have thedecomposition effect on the starch. Pseudomonas, Bacillus, Enterobacteriaceae have the decomposition effect on the protein. Cellulose only can be decomposed by the Cellulomonas.Lactpbacillus, Bacillus, Sporolactobacillus can break up the fat.(5) Study of ecological correlation test shows that there is no antatonism among the aboriginalbacteria.(6) Regressive Tests of the effective bacteria shows that combined strains can accelerate thecompost. So we can add the effective microbes at the beginning of the compost or at differentphases based on the characteristics of the microbes.