Studies On Feed Enzyme Preparation Deriving From Cultural Residue Of Edible Fungi

In order to make use of wasted cultural residue of edible fungi rationally, it wasstudied on the exploiting of feed enzyme preparation taking it as raw material. It wasalso discussed on the effect of feeding animals with cultural residue of edible fungifrom the perspective of enzyme.In this research, the activities of cellulase, xylanase,α-galactosidase and pectasewere detected by DNS method, and the activity of proteinase was measured by Folinmethod, and the activity of phytase was by another method. Then the activities ofcellulase, xylanase,α-galactosidase, pectase, proteinase and phytase were detectedrespectively in several kinds of cultural residue of edible fungi, so the optimal onecould be screened by comparison. Then the enzyme solution was concentrated bydifferent means and the precipitate was dialysed, and the tmwatered enzymes wereabtained by vacuum drying. In the course of the experiment, part of the zymologicproperty was studied. The main results and conclusions were as follows:1. There were merits of simple operation, high sensitivity and good repeatabilitymeasuring the activities of cellulase, xylanase,α-galactosidase and pectase withDNS method.2. Several kinds of enzymes were detected in the cultural residue of Xianggu,Fengweigu, Jigu, Xiuzhengu, Jinzhengu, and it was determined that the culturalresidue of Xianggu was the optimal cultural residue of edible fungi for exploiting feedenzyme preparation. In addition, the effectiveness of feeding animals with culturalresidue of edible fung was explained from the perspective of enzyme.3. The optimal extraction method that cultural residue of edible fungi was extractedwith distilled water for 12 hours at 10℃was determined; Ammonium sulfate settingmethod was determined to concentrate the enzyme solution, after concentration, theactivity of cellulase was 9～13 times of that of the original enzyme solution, and themaximum enzyme activity was up to 217.24IU/g. The activity of xylanase was 6～28times of that of the original enzyme solution, and the maximum enzyme activity wasup to 398.50 IU/g; The dry enzyme preparation was obtained, and the activity of cellulase in it was up to 80.21 IU/g, and the activity of xylanase was up to 144.83IU/g. So it was proved that there was potential value for the cultural residue ofXianggu exploiting feed enzyme preparation.4. The optimal pH of cellulase was 4, and optimal temperature was 55℃.When thepH was between 3～5 and the temperature was below 65℃, the stability of thisenzyme was high; The optimal pH ofxylanase was 4, and optimal temperature was 45℃.When the pH was between 3～6 and the temperature was below 70℃, the stabilityof this enzyme was high.