Identification And Expression Analyses Of Cold-regulated Genes On Citrus

Citrus origin in china, which is one of the most important fruit in the world, ranksthe number one of the world's fruit production. It is the Main fruit in China's YangtzeRiver Valley and south province of the Valley, and has become one of the pillarindustries of the rural economy in south China. Freezing damage is one of the majordisasters of the world citrus production. Historically, citrus-producing areas in YangtzeRiver Valley, Florida and California in United States suffered several serious cyclicalfreezing damages. Cold resistance breeding has become one of the main elements of thecitrus producing breeding in the main producing countries in the world. People throughtraditional breeding methods to improve citrus cold hardiness, but because of the coldhardiness of citrus mechanisms are poorly understood and little progress has been madeso far.This research used suppression subtractive hybridization (PCR-Select cDNAsubtraction) technology to analyze expression of cold resistance related Genes onPoncirus trifoliata Raf. Identified and cloned the differentially expressed cDNA tosubtractive library can be identified, won a number of unknown Citrus uniquecold-related genes. And finished detection means Improvement of kit application ofCitrus in the course of the study.The experiment of low temperature test of differentcitrus in growing period has developed an optimum low-temperature processingconditions of the growing citrus. Based on this result, the preliminary expressionanalysis of PM-type Ca²⁺-ATPase gene and citrus cold accumulate protein codinggene has been done.Lay the foundations for Cold-resistance genetic engineeringbreeding of citrus. Provide a theoretical basis for the research of the cold hardinessmolecular mechanisms on citrus.