Natural Product Chemistry Study On Fruiting Body Of Hericium Caput-medusae (Bull.:Fr.) Pers

The fungi in genus Hericium are valuable edible and medical fungi. H. caput-medusae(Bull.:Fr.) Pers., belonging to the genus Herieium, is a special product of Changbai Mountain inJilin Province. Based on the research achievements of the other researchers, we made a furtherstudy and probe into the chemical compositions in the fruiting body of H. caput-medusae.The fungi in genus Hericium are nutritious, abundant in many kinds of vitamins, amino acidsand trace elements, they are of great value not only in nutrition aspect, but also in medical aspect.Up to new, the research reports on Hericium both in domestic and abroad have focused mainly onpolysaccharides components, which have been proved by biological and pharmacologicalresearches to have the effects on improving immunity, restraining cancer, reducing blood pressureand blood sugar, and so on, while researches on their small molecular components were relativelyless and not deepgoing. Modern medical and pharmacological researches showed that smallmolecular substances as sterols have remarkable effect on deducing cholesterol, restraining cancer,arresting oxidization, resisting inflammation, prophylaxis and treatment of the prostate diseases,while terpenes as nerve factor stimulator can remedy Senile Dementia. Thus it is of great realisticsignificance to carry out deepgoing researches on chemical compositions, especially smallmolecular substances, in H. caput-medusae.Normal phytochemistry methods and technology such as organic solvent extraction, twophases solvent extraction, amberlyst chromatography, silica gel column chromatography andcrystallization, combined with advanced instruments and apparatus, were applied in thisexperiment, and five monomer compounds were extracted and isolated through different routes.On the basis of physical constants, chemical evidence and spectrum analysis [NMR, MS], twomonomer compounds were identified, both of which were ergosterins: compound 2 wasErgosta-4-en-1,3,6-triol, (22Z)- and compound 4 was Ergosta-5,7,22-trien-3-ol, (22Z)-. Thesetwo sterol compounds were the first report to be extracted and isolated form H. caput-medusaeand they were both new natural compounds. There isolation added new contents to thefundamental research and provided new foundation for it.In addition, we used SEF-CO₂ extraction technology, combined with GC/MS technology inthis experiment, and isolated and identified 27 compounds from Liberation kettleⅠ和Ⅱ. These compounds can be divided into three categories: stearin acid esters, diolefines and nitrogenouscompounds. These compounds not only make the chemical composition research results in H.caput-medusae more abundant, but also do the groundwork for the pharmacological researches onH. caput-medusae in the future.In conclusion, we did some foundamental work in studying the chemical compositions in H.caput-medusae,. Our results provide theory basis for correctly evaluate the medical value of H.caput-medusa, add to new contents to the pharmaceutical range of H. caput-medusae as well asthe number of ergosterins in nature, and make the basis for new medicine exploitation from H.caput-medusae.