Establishment Of In Vitro Propagation Systems Of Dracaena Fragrans And Study On Variation Of Chimera Variety

Dracaena fragrans (L. ) Ker—Gaw, originating from Guinea, which has developed into a major potted foliage plants in recent years, The Pearl River Delta is the potted seedlings and concentrate production of Dracaena fragrans in China.The experimental made a study of the in vitro culture system of Dracaena fragrans,in vitro process of variation of chimera varieties and lower mutation rate of technical measures for a series of studies.Firstly, Take the stem section of three varieties as a material,this paper makes a study of the influence of the factors such as the plant growth regulators, the different basal culture medium, silver nitrate, the cultivation medium upon tissue culture regeneration. The results showed that the axillary buds germination grows around and at the same time part axillary buds base stem section organization's differentiation also was initiated with MS+3.0mg/L BA+0.3mg/L IAA+2.0mg/L AD; then raises one generation for MS+3.0 mg/L BA+0.3 mg/L IAA, which 5-6 adventitious buds were split up from each explants about 1mm equally; the proliferation rate of adventitious bud is over 390.00 % for MS + 1.0mg/L BA+0.1mg/L IAA+3.0 mg/L AgNO₃, The adventitious buds which is bigger than 1.2cm raises one generation on the strong sprout to MS+0.05 mg/L BA+0.05 mg/L IAA+ 1.0%AC, the bud highly increases approximately above 1.5 times with coarseness, average high approximately 3.5cm;The sprout which is higher than 3 cm change over 1/2MS+0.5mg/L IBA to induce rooting;Finally the seedling grows forty-five days when moves to the coconut tree rice polishings: Marly soil: On the pearlite (1:1:1),which three seedlings is high separately above 15, 16, 10 cm. Through more than tow years of production practice examination, established a set highly effective and stable tissue culture system of Dracaena fragrans , its initiation rates,the bud differentiation rate, the rooting rates,the survival rates was above 98% stably.Secondly,take two chimaera variety of Dracaena fragrans as the material, its variation of leave chimera character during in vitro culture and reduce its variation rate were studied. Two varieties of leave chimera character in vitro extremely unstable, the massangeana has 4 kind of variation, the lindenii has 8 kind of variation; Their main variation rate of regeneration bud by the way, from callus tissue differentiation adventitious buds of the mutation rate is over 70%, from numerous basal bud differentiation of adventitious buds variation rate approximately 40% to 50%, and divide by the axillary buds form the bud mutation rate of only 13% to 17%, but only forms of proliferation bud proliferation rate is too low. Where can inhibit callus formation and differentiation, and to promote germination bud differentiation and technical measures can enhance chimera traits stability in vitro culture. Therefore, we can choose culture materials through the appropriate medium to reduce the growth regulator used and the addition of 3.0mg / L AgNO₃ to reduce variability occurred, maintains the higher multiplication efficiency. That is the base of meristem with the numerous buds (10 Proliferation generation, from a single chimera Bud) materials, That is the base of meristem with the numerous buds (to multiply 10generations, takes single embedment bud) as materials,and the proliferation of culture on MS+1.0 mg/L BA + 0.1 mg/L IAA+ 3mg/L AgNO₃ , two varieties of mutation rates were 22.80% and 25.34, respectively, the effective proliferation rate were 355.02% and 296.58%.