Studies On The Establishment Of In Vitro Rapid Propagation Systems Of Two Species Of Ornamental Ferns

In vitro culture of Osmunda japonica and Aleuritopteris argentea were studied inthis project. Spores, prothallus and rhizome were used as explants for the culture. TheGGB(green globular bodies) of Aleuritopteris argentea, and the GGB and callus ofOsmunda japonica were successfully induced in the different culture conditions, and thedifferent media with different types of hormone concentrations and the mix of differenttests. In vitro propagation systems of two fems were established, which providedimportant data for the industrialized propagation in macro and exploitation of these ferns.The main results were as follows:Using rhizome of Osmunda japonica as explant, 1/2MS medium containing 0.1mgl^-1BA and sucrose 30gl^-1 was the appropriate medium for inducing GGB. After 40 daysof culture, the biggest multiplication coefficient was 27.50, and the induction rate of GGBwas 100%. The appropriate medium for GGB differentiation is: 1/2MS with30gl^-1 sucrose.GGB differentiation rate was 100%. The suitable medium for plantletsrooting was: 1/2MS+sucrose 20gl^-1+NAA 0.1mgl^-1. Rhizomes removed off all leaveswere the best explant for callus induction. The suitable medium for callus induction was:1/2MS+2, 4-D 3.0mgl^-1. The culture condition of callus induction was the normalillumination(illumination intensity 1200-14001x). The medium of 1/2MS containing0.1mgl^-1 NAA was optimal for differentiation of callus of Osmunda japonica. Thedifferentiation coefficient was 5.433. The suitable transplanting substrate for plantletswith roots was the mixture of leaf mold and perlite (the ratio of 2:1)and the survival ratiowas 47.5%.The Aleuritopteris argentea spores were filtrated after oscillating with 2% NaClOsolution for 8-10 minutes, and the contamination rates can be lowered to 0. This was theoptimal disinfection method for Aleuritopteris argentea spores. The suitable cultureconditions for spores germinating were normal illumination(illumination intensity1200-14001x). And the medium was MS together with 20-40gl^-1 sucrose. The sporesgermination rate was over 70%. Using prothallus as explants, GGB can be induced after50 days of culture. And the optimal medium was MS containing 1.0-2.0mgl^-1 BA together with 0.3 mgl^-1 NAA. The multiplication coefficient was up to above 9 after 50 days. Thesuitable medium for GGB differentiation was MS+KT 0.1mgl^-1+NAA 0.1mgl^-1 and thedifferentiation rate was 100%. The average plant leaf of differentiation of was six. Thesuitable medium for plantlets rooting was: MS+sucrose 20gl^-1+IBA 0.5mgl^-1. Thesuitable transplanting substrate for plantlets with roots was the mixture of leaf mold andperlite (the ratio of 2:1) and the survival ratio was 75%.