Study On The Effects Of Two Derivates Of Glycine On The Bacteria-inhibiting In Vitro,Toxicological And The Production Performance Of Broiler

The in vitro antibacterial activity of salicylaldehyde-glycine ligands and its complex with Cu(II) was studied and acute toxicological test on the complex with Cu(II) was conducted.Finally, the effects of the two derivates of glycine replacing CTC in diet on production performance, carcass characteristics and some immunity index of broiler was studied to evaluate the possibility of the complex with Cu(II)replacing CTC in diet of broiler.The salicylaldehyde- glycine and its complex with Cu(II) were synthesized. The antibacterial activity of this ligands and complexes against four bacteria (E.coli, S.aureus, sh.lexneri, S.typhimurium) was determined by using concentration dilution method. The results showed that antibacterial activity of the salicylaldehyde-glycine and its complex was stronger against G+(S.aureus)and G-( E.coli, sh.lexneri, S.typhimurium). Complexes are stronger than ligands.One hundred and twenty mice of Kunming breed, about 18~22g of live weight, half of female and male, were used in this experiment. they were divided into control, test groups of five (with 20 mice in each group). The animals were poured 80% of dimethylsulfoxide through mouth and the mice in test groups were poured 80% of dimethylsulfoxide and Complexes respectively. The result showed that the LD50 =1271.7 mg/kg of complex with Cu(II).Three hundred 1-day-old AA+ broiler chickens were randomly divided into control group and test groups of I, II, III and IV. The control group I was fed diet. The control groupII was fed diet with CTC.Broilers in the test group I ,II , III and IV were fed diet replacing CTC with (2,1.5,1,0.5g/kg)(0~2 weeks) and (1,0.75,0.5,0.25g/kg)(4~6 weeks)of Salicylaldehyde-glycine complex with Cu(II) diet respectively. The results showed that average daily gain of birds in group I, II, III, IV and V was increased by-0.71%,2.76%,10.67%(P<0.05),4.19%;Feed Conversation ratio was not divergent in control group and test groups of I, II, III,IV and V. the Salicylaldehyde-glycine complex with Cu(II) improved the growth performance, brisket rate, leg portion rate, the indexes of immune organs;decreased abdomen fat of broilers on the 42nd day to some degrees. It showed that the effect of test group III were equal with the control groupII, test group III was the best group.According to NY/T843-2004 and (GB15199-94)Cu in chicken tissues≤10 mg/kg, Detected Cu in chicken tissues (liver tummy , kidney, bone , brisket , leg portion) The results showed that the test group III and IV were all not over the standards ,They were proper test group.In conclusion, the two derivates of glycine had property of inhibiting bacteria. Replacing antibiotics with the complex in broiler diet is practicely and supplementation with the levels of 1,0.5g/kg(0~2 weeks) and 0.5,0.25g/kg (4~6 weeks) had the best effect on broiler.