Study On In Vitro Culture And In Vitro Flowering Of Dianthus Caryophyllus L.

In vitro,in vitro flowering and internal variation of physiological and biochemical during flower bud differentiation for Dianthus caryophyllus L.through tissue culture, can build up Dianthus caryophyllus L.system of intermediate propagation,decrease the juvenile phases from a vegetative growth to a reproductive growth stage,inquiry into flower bud differentiation regulation is significance not only in theory but also in application.The objective of this research is to study the effects of differ culture medium, 6-BA,NAA,differ Agar and closure film during the induction of lateral bud of Dianthus caryophyllus L..The results of our research show that the contrast of differ 6-BA and NAA concentration is very remarkable on the induction rate of lateral bud and leaf,also the effects of differ 6-BA and NAA concentration go together with ratio combination are very difference.When 6-BA and NAA are combined in level of 2.0 mg.L^-1and 0.5 mg.L^-1,the induction rate of lateral bud is highest at 87.9%and 11.2%. The effect of differ Agar of culture medium is very remarkable on the induction of lateral bud and leaf of Dianthus caryophyllus L.As its Agar is 7g.L^-1,the induction rate is highest,bud grow best and grow many leafs.The effect of differ closure film of culture medium is very remarkable on the induction of lateral bud and leaf of Dianthus caryophyllus L.Through using closure film,the effect on the induction of lateral bud of Dianthus caryophyllus L.also is significance,results shows that closure film can make bud grow rapidly and become a plant. The mostly objective of this research is to study the effects rooting,callus,N/P, sucrose,6-BA and NAA concentrations on flowering in vitro Dianthus caryophyllus L.. The results of our research show that the plant takes root to repress flower bud inducement obviously,it is not very impact of floral bud induction whether differentiate callus or not.The results showed that the floral bud induction rate was obviously promoted when we adjusted the N of ammonium nitrate and P content,one of N/P ratio is 1N and 5P(phosphor concentration)can make highest rate of floral bud induction.N and P ratio of contention influence the floral color depth,and the normal growth of plant and leaf.When 6-BA and NAA are combined in level of 0.5mg.L^-1and 0.1 mg.L^-1,the induction rate of flowering is highest at 72.3%.The optimal way for inducing floral buds is to preculture the plantets on new MS medium added 35g.L^-1 sucrose,7g.L^-1agar,0.5mg.L^-16-BA and 0.1mg.L^-1NAA.Plantlets,which were new stem growth from subcultured plantlets,were induced to from floral buds in 60 days. The rate of flower induction was about seventy percent.The optimal medium for inducing floral buds is new MS medium added 35g.L^-1 sucrose,7g.L^-1agar,0.5mg.L^-16-BA and 0.1m NAA.The inefficient medium for inducing floral buds is MS medium added 30g.L^-1sucrose,6.5g.L^-1agar.This paper still study on the variation and correlations of physiological and biochemical of Dianthus caryophyllus L.during the flower bud induction.The results showed that the contention of soluble proteins,soluble sugar and incretion were higher in control group which floral bud induction is high than in treatment group which floral bud induction is low.